Summary of Study ST003040

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001892. The data can be accessed directly via it's Project DOI: 10.21228/M8572M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003040
Study Title	MM.1S Myeloma tumor cells and tumors made by subcutaneously injecting 1M Luc+/GFP+ MM.1S cells treated with 50 μM BMS309403 and Vehicle (PBS with a matched concentration of 5% DMSO)
Study Summary	MM.1S Myeloma tumor cells were treated with 50 μM BMS309403 once over 24 hours in vitro or at 5mg/kg X3 per week over 32 days in vivo. In Vivo tumors were made by subcutaneously injecting 1M Luc+/GFP+ MM.1S cells mixed with Matrigel in a 1:1 ratio into the backs of 8-week old, female SCID-Beige. Vehicle (PBS with a matched concentration of 5% DMSO) was used as control for each condition.
Institute	Mainehealth
Last Name	Reagan
First Name	Michaela
Address	81 Research Drive, Scarborough, ME, Portland, ME, 04074, USA
Email	Michaela.Reagan@mainehealth.org
Phone	(207)396-8196
Submit Date	2023-12-20
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2024-03-25
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003160
Sampleprep Summary:	Lipids were extracted from cell and tumors previously treated with Vehicle and BMS309403 using a modified Bligh and Dyer protocol [Bligh and Dyer, 1959] of Avanti Polar Lipids, INC. Water:Methanol:Dichloromethane (34:34:31, v/v/v). After the extraction and dried out the samples were resuspended in Methanol:Dichloromethane (50:50, v/v) with 10 mM of Ammonium acetate to be injected by Direct Infusion.

Sampleprep ID:

SP003160

Sampleprep Summary:

Lipids were extracted from cell and tumors previously treated with Vehicle and BMS309403 using a modified Bligh and Dyer protocol [Bligh and Dyer, 1959] of Avanti Polar Lipids, INC. Water:Methanol:Dichloromethane (34:34:31, v/v/v). After the extraction and dried out the samples were resuspended in Methanol:Dichloromethane (50:50, v/v) with 10 mM of Ammonium acetate to be injected by Direct Infusion.