Summary of Study ST000646

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000460. The data can be accessed directly via it's Project DOI: 10.21228/M8M027 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000646
Study Title	Effects of Exercise on Dystrophic Mouse Muscle TCA Cycle (part II)
Study Summary	We will use male C57BL10, mdx, and nNOS-/- mice (n = 10 each group) to characterize the skeletal and cardiac muscle metabolomes. Half of the mice in each group will remain sedentary while the other half will be subjected to a single bout of treadmill exercise to exhaustion. Mice will be euthanized immediately postexercise and blood, hearts, and hindlimb muscles will be harvested and frozen as detailed in the General Methods. The heart and gastrocnemius, soleus, and quadriceps muscles of one hindlimb will be sent to the Mayo Clinic Metabolomics Resource Core. The heart and quadriceps muscle will be used for untargeted metabolomics profiling (LC/MS) while the gastrocnemius and soleus muscles will be used for targeted analyses of amino acids plus amino metabolites, non-esterified fatty acids, and citric acid cycle intermediates.
Institute	Mayo Clinic
Last Name	Thomas
First Name	Gail
Address	Penn State Hershey Heart and Vascular Institute Penn State College of Medicine 500 University Drive, MC H047 Hershey, PA 17033
Email	gthomas4@hmc.psu.edu
Phone	717-531-0003, ext. 287087
Submit Date	2017-06-23
Analysis Type Detail	GC-MS
Release Date	2019-07-17
Release Version	1

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Treatment:

Treatment ID:	TR000683
Treatment Summary:	Studies will be performed in 12-16 week old male mdx mice, C57BL10 control mice, and nNOS-/- mice obtained from Jackson Laboratory. All protocols will be approved by the Penn State College of Medicine Institutional Animal Care and Use Committee. Treadmill Exercise: Mice will run on a horizontal treadmill (Columbus Instruments) to assess fatigue by a single bout of exercise beginning at 5 m/min for 5 min followed by 1 m/min increases every minute until exhaustion. Electric shocks will not be used to stimulate running due to adverse effects in mdx mice.

Treatment ID:

TR000683

Treatment Summary:

Studies will be performed in 12-16 week old male mdx mice, C57BL10 control mice, and nNOS-/- mice obtained from Jackson Laboratory. All protocols will be approved by the Penn State College of Medicine Institutional Animal Care and Use Committee. Treadmill Exercise: Mice will run on a horizontal treadmill (Columbus Instruments) to assess fatigue by a single bout of exercise beginning at 5 m/min for 5 min followed by 1 m/min increases every minute until exhaustion. Electric shocks will not be used to stimulate running due to adverse effects in mdx mice.