Summary of Study ST001348

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000917. The data can be accessed directly via it's Project DOI: 10.21228/M8568J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001348
Study Title	Metabolomic changes in mouse liver on a threonine restricted diet (part-III)
Study Summary	The data provided here are in support of the publication “Restriction of essential amino acids dictates the systemic metabolic response to dietary protein dilution” Yann W. Yap et al. Nature Communications 2020 (accepted for publication). The data in this study corresponds to liver from C57Bl/6JMarp mice subject to a 3wk treatment with diets either containing 18% digestible energy from amino acids with either a normal distribution of amino acids or with restricted amounts of threonine. This followed prior treatment with adeno-associated viruses to transduce the liver to express yeast threonine biosynthetic enzymes (AAV-yTHR1+THR4) or a negative control (AAV-GFP). n= 6 individual mice per group.
Institute	Monash University
Department	Department of Biochemistry and Molecular Biology
Laboratory	Nutrient Metabolism and Signalling Laboratory
Last Name	Rose
First Name	Adam
Address	Monash University, Clayton Campus, 23 Innovation Walk, Clayton, Victoria, 3800, Australia
Email	adam.rose@monash.edu
Phone	+61 3 99029340
Submit Date	2020-04-03
Num Groups	4
Total Subjects	24
Num Males	24
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2020-07-20
Release Version	1

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Treatment:

Treatment ID:	TR001437
Treatment Summary:	Male mice aged 7 weeks upon arrival, were acclimated to the local housing facility (12-12h light-dark cycle, 22-24°C) for one week prior to experimentation and were fed standard rodent chow (8720610, Barastoc, AUS). Following acclimation, mice were administered a total of 5E+11 virus particles per mouse via the tail vein. For the negative control (NC): 5E+11 GFP-AAV; and for the THR1/4 overexpression studies mice were administered 2.5E+11 virus particles each of yTHR1-AAV and yTHR4-AAV. One week following this time, the dietary intervention was initiated. In these studies, the low threonine diet (SF18-066, Specialty Feeds AUS) contained homoserine, the substrate of THR1. The corresponding control diet was SF17-177 (Specialty Feeds AUS).

Treatment ID:

TR001437

Treatment Summary:

Male mice aged 7 weeks upon arrival, were acclimated to the local housing facility (12-12h light-dark cycle, 22-24°C) for one week prior to experimentation and were fed standard rodent chow (8720610, Barastoc, AUS). Following acclimation, mice were administered a total of 5E+11 virus particles per mouse via the tail vein. For the negative control (NC): 5E+11 GFP-AAV; and for the THR1/4 overexpression studies mice were administered 2.5E+11 virus particles each of yTHR1-AAV and yTHR4-AAV. One week following this time, the dietary intervention was initiated. In these studies, the low threonine diet (SF18-066, Specialty Feeds AUS) contained homoserine, the substrate of THR1. The corresponding control diet was SF17-177 (Specialty Feeds AUS).