Summary of Study ST002079
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001319. The data can be accessed directly via it's Project DOI: 10.21228/M87125 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002079 |
| Study Title | Defining the mammalian coactivation of hepatic 12-hour clock and lipid metabolism |
| Study Summary | The 12-hour clock coordinates lipid homeostasis, energy metabolism and stress rhythms via the transcriptional regulator XBP1. However, the biochemical and physiological basis for integrated control of the 12-hour clock and diverse metabolic pathways remains unclear. Here, we show that steroid receptor coactivator SRC-3 coactivates XBP1 transcription and regulates hepatic 12-hour cistrome and gene rhythmicity. Mice lacking SRC-3 show abnormal 12-hour rhythms in hepatic transcription, metabolic functions, systemic energetics, and rate-limiting lipid metabolic processes including triglyceride, phospholipid and cardiolipin pathways. Notably, 12-hour clock coactivation is not only preserved, with its cistromic activation priming ahead of the zeitgeber cue of light, but concomitant with rhythmic remodeling in the absence of food. These findings reveal that SRC-3 integrates the mammalian 12-hour clock, energy metabolism, and membrane and lipid homeostasis, and demonstrates a role for the 12-hour clock machinery as an active transcriptional mechanism in anticipating physiological and metabolic energy needs and stresses. |
| Institute | Baylor College of Medicine |
| Last Name | Meng |
| First Name | Huan |
| Address | One Baylor Plaza BCM 130 Houston, TX 77030 |
| huanm@bcm.edu | |
| Phone | 5127729532 |
| Submit Date | 2022-02-04 |
| Num Groups | 12 |
| Total Subjects | 24 |
| Num Males | 24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | Other |
| Release Date | 2022-02-22 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR002174 |
| Treatment Summary: | Mouse liver lipids were extracted using a modified Bligh-Dyer method. Briefly, 50 mg of crushed issue sample from mouse whole liver was used. A 2:2:2 volume ratio of water/methanol/dichloromethane was used for lipid extract at room temperature after spiking internal standards 17:0 LPC, 17:0PC, 17:0 PE, 17:0 PG, 17:0 ceramide, 17:0 SM, 17:0PS, 17:0PA, 17:0 TAG, 17:0MAG, DAG 16:0/18:1, CE 17:0. The organic layer was collected followed by a complete drying procedure under nitrogen. |
