Summary of Study ST002252
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001440. The data can be accessed directly via it's Project DOI: 10.21228/M8KQ6P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002252 |
| Study Title | Lipidomics analysis on Arabidopsis autophagy mutants |
| Study Summary | Autophagy is an essential cellular process in eukaryotes that degrades and recycles macromolecules and organelles. Defects in autophagy is known to affect metabolism, including the lipidome. Genetic approaches have identified a series of AuTophaGy-related (ATG) genes in Arabidopsis. In this study we used WT (ecotype Col-0) and two Arabidopsis autophagy-defective mutants, atg7 and atg9 to perform a multi-omics study on the effect of nitrogen starvation treatment, which induces autophagy. Specifically, we have quantified ~100 lipids from leaf and root tissues of WT, atg7 and atg9 mutant plants, under either autophagy-inducing conditions (-N) or normal nitrogen conditions (+N). The lipid species we quantified include: DGDG, MGDG, LPC, LPE, PE, LPG, PC, PA, PG, PI, and PS. Our study sheds lights on the understanding of the relationships between autophagy and metabolism, especially lipid metabolism. |
| Institute | Iowa State University |
| Department | Biochemistry Biophysics, and Molecular Biology |
| Laboratory | Nikolau Lab |
| Last Name | Ding |
| First Name | Geng |
| Address | 2252 Molecular Biology BLDG, Pammel Drive |
| gengding@iastate.edu | |
| Phone | 515-294-0347 |
| Submit Date | 2022-02-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2023-02-21 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR002350 |
| Treatment Summary: | Plants were grown in either normal or nitrogen deficient media for last 3 days. -N condition was used for inducing of autophagy processes. |
| Treatment: | Nitrogen deficient media |
| Plant Light Period: | 24 hr |
| Plant Humidity: | 100% (Enclosed hydroponic growth condition) |
| Plant Temp: | 22 C |
| Plant Growth Stage: | 11-days |
