Summary of Study ST002919
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001814. The data can be accessed directly via it's Project DOI: 10.21228/M87D9M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002919 |
Study Title | Short-term metabolic insulin response of DINCH- and MINCH-treated cells |
Study Summary | In the second part of the project, we examined the short-term metabolic insulin response of DINAH- and MINCH-treated cells and compared them with rosiglitazone-differentiated cells. For this purpose, the human SGBS preadipocyte cell line was exposed to differentiation media conditioned with DINCH (10 nM or 10 µM), MINCH (10 nM or 10 µM), or rosiglitazone, and the insulin response was measured by analyzing the changes in glycolysis and PPP between insulin-stimulated and non-insulin-stimulated cells. In conclusion, the insulin response of glycolysis and PPP of cells treated with 10 µM MINCH, but not with 10 nM MINCH or DINCH, was similar to cells differentiated with rosiglitazone. |
Institute | Helmholtz Centre for Environmental Research |
Last Name | Engelmann |
First Name | Beatrice |
Address | Permoserstr. 15 |
beatrice.engelmann@ufz.de | |
Phone | 00493412351099 |
Submit Date | 2023-10-08 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2023-11-01 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR003041 |
Treatment Summary: | SGSB preadipocytes were maintained at 37°C and 5% CO2 in 95% humidity. To examine the insulin response of SGBS treated with DINCH and MINCH, SGBS preadipocytes were treated for 18 days with differentiation media without the PPARG agonist rosiglitazone supplemented with DINCH or MINCH (10 nM and 10 µM). To obtain an adipogenesis reference, SGBS cells were differentiated with rosiglitazone for 18 days according to the standard differentiation protocol. After replacing the medium on day 17 with differentiation medium without insulin and overnight incubation (insulin starvation), cells were starved for 1 hour with differentiation medium without glucose and insulin (glucose + insulin starvation). Subsequently, cells were incubated with either differentiation medium with insulin (insulin-stimulated cells correspond to plus-insulin samples) or without insulin (unstimulated cells correspond to minus-insulin samples). During differentiation, a final concentration of 0.01% (v/v) MeOH and 0.02% (v/v) DMSO was added to all differentiation media. Continuous exposure was mimicked by replacing the cell culture medium every other day. Each treatment was performed in five biological replicates (n=5). |