Summary of Study ST002765

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001723. The data can be accessed directly via it's Project DOI: 10.21228/M80F0C This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002765
Study TitleDisorder of Lipids Induced by Gestational Asthma and its Effect on the Development of Fetal Lung Function and Fetal Health
Study SummaryMaternal asthma during pregnancy is highly correlated with fetal growth and development, and can cause damage to both the mother and fetus, but the underlying mechanisms are not yet clear. Amniotic fluid, as the environment for fetal growth and development, may be affected by lipid metabolism disorders, which can impact fetal lung function development. A rat model of asthma during pregnancy induced by common allergen house dust mite (HDM) was used to investigate changes in lipid composition in amniotic fluid and bronchoalveolar lavage fluid (BALF) by ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS), revealing the impact of maternal asthma during pregnancy on fetal lipid metabolism. In this study, maternal asthma aggravated inflammatory indicators and pathological manifestations after fetal allergen exposure, creating a high oxidative stress growth environment for the fetus, and causing metabolic differences in various lipid groups, including phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and fatty acids (FA), indicating significant lipid metabolism disorders. Improving lipid metabolism may help asthmatic pregnant women maintain healthy fetal development.
Institute
Nanjing University of Chinese Medicine
Last NameFang
First NameHuafeng
AddressNo.138 xianlin road, nanjing city, Nanjing, China, 210046, China
EmailRiorofhf@outlook.com
Phone+86 18852416998
Submit Date2023-06-29
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-01-01
Release Version1
Huafeng Fang Huafeng Fang
https://dx.doi.org/10.21228/M80F0C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001723
Project DOI:doi: 10.21228/M80F0C
Project Title:Lipid metabolism affects fetal development
Project Summary:Gestational asthma interferes with lipidomic metabolism of amniotic fluid and fetal alveolar lavage fluid, thus inhibiting fetal development
Institute:Nanjing University of Chinese Medicine
Last Name:Fang
First Name:Huafeng
Address:No.138 xianlin road, nanjing city, Nanjing, China, 210046, China
Email:Riorofhf@outlook.com
Phone:+86 18852416998

Subject:

Subject ID:SU002872
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA292140PBS1Control
SA292141PBS7Control
SA292142PBS6Control
SA292143PBS2Control
SA292144PBS3Control
SA292145PBS4Control
SA292146HDM8HDM
SA292147HDM7HDM
SA292148HDM3HDM
SA292149HDM4HDM
SA292150HDM5HDM
SA292151HDM6HDM
Showing results 1 to 12 of 12

Collection:

Collection ID:CO002865
Collection Summary:Amniotic fluid was extracted from the amniotic membrane of GD18 gestational rats and stored at -80℃
Sample Type:Amniotic fluid
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002881
Treatment Summary:In the sensitization group, the sensitization solution was injected intritoneally every 2 days before pregnancy, with 100ul of sensitization solution injected each time, equivalent to 20ug of HDM injection, for a total of 3 times. The control group was given the same amount of PBS injection. Use of HDM nasal drops during pregnancy

Sample Preparation:

Sampleprep ID:SP002878
Sampleprep Summary:500 μL of amniotic fluid was lyophilized and redissolved in 200 μL of deionized water. Then, 80 μL of redissolved amniotic fluid was pipetted off into a 1.5 mL centrifuge tube containing 225 μL of ice-cooled methanol (Merck, Germany) pre-mixed with lyso PE (17:1; LM171LPE-11), SM (17:0; 170SM-13), and PE (17:0/17:0; LM170PE-19) internal standards (5 μg·mL-1; Avanti Polar Lipids, USA). The solution was vortexed for 10 s and added with 750 μL of ice-cooled MTBE. The mixture was shaken for 10 min at 4 °C, added with 188 μL of deionized water, vortexed for 20 s, and centrifuged at 18000 rpm for 2 min at 4 °C. 350 μL of the upper layer (the organic phase, mainly including lipids) and 110 μL of the bottom layer (the aqueous phase, mainly including polar substances) were separately transferred to a new centrifuge tube (1.5 mL). The samples were dried using the Savant SPD1010 vacuum centrifugal concentrator (Thermo Fisher Scientific, USA) and stored at -20 °C before testing. Lipids in the upper layers were lysed with 110 μL of methanol-toluene (9:1) solution.

Combined analysis:

Analysis ID AN004500 AN004501
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo UltiMate 3000 RS Thermo UltiMate 3000 RS
Column Waters Acquity UPLC CSH C18 column (100 mm×2.1mm, 1.7 m) Waters Acquity UPLC CSH C18 column (100 mm×2.1mm, 1.7 m)
MS Type ESI ESI
MS instrument type Other Other
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH003381
Instrument Name:Thermo UltiMate 3000 RS
Column Name:Waters Acquity UPLC CSH C18 column (100 mm×2.1mm, 1.7 m)
Column Temperature:65℃
Flow Gradient:15% B at 0 min, 30% B at 0–2 min, 48% B at 2–2.5 min, 82% B at 2.5–11 min, 99% B at 11–12 min and 15% B at 12–15 min.
Flow Rate:0.6 mL/min
Solvent A:60%acetonitrile;40%water
Solvent B:90%isopropanol;10% acetonitrile;0.1% formic acid;10mM ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS004247
Analysis ID:AN004500
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Other
MS Type:ESI
MS Comments:Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer was used for both positive and negative ion modes. Parameters of mass spectrometry: spray voltage was 3.5 kV (positive) and 3.0 kV (negative); for both ionization modes, sheath gas, aux gas, capillary temperature, and heater temperature were maintained at 35 arb, 15 arb, 325 °C and 300 °C, respectively; scan range was m/z 215–1800.
Ion Mode:POSITIVE
  
MS ID:MS004248
Analysis ID:AN004501
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Other
MS Type:ESI
MS Comments:Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer was used for both positive and negative ion modes. Parameters of mass spectrometry: spray voltage was 3.5 kV (positive) and 3.0 kV (negative); for both ionization modes, sheath gas, aux gas, capillary temperature, and heater temperature were maintained at 35 arb, 15 arb, 325 °C and 300 °C, respectively; scan range was m/z 215–1800.
Ion Mode:NEGATIVE
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