Summary of Study ST002918

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001799. The data can be accessed directly via it's Project DOI: 10.21228/M85M79 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples  |  Perform analysis on untargeted data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002918
Study TitleMetabolic Profiling in mouse Infected with Vibrio parahaemolyticus
Study SummaryMice were subject to intraperitoneal (i.p.) injection with the V. parahaemolyticus, and control mice were injected with saline. Data were collected and analyzed using unsupervised hierarchical clustering. In general, the abundance of metabolites increased more often than it decreased in animals with higher resistance to infection. Load weight analysis of biological pathways suggested that alanine, aspartate, glutamate metabolism could play roles in the capacity of mice to survive infection with V. parahaemolyticus.
Institute
Sun Yat-sen University
Last Namejiang
First Nameming
AddressNo. 135, Xingang Xi Road, Guangzhou, 510275, P. R. China
Emailjiangm28@mail.sysu.edu.cn
Phone13434283781
Submit Date2023-09-16
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2023-10-05
Release Version1
ming jiang ming jiang
https://dx.doi.org/10.21228/M85M79
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001799
Project DOI:doi: 10.21228/M85M79
Project Title:Exogenous L-Alanine promotes phagocytosis via dual regulations of TLR4 to eliminate multidrug-resistant bacterial pathogens
Project Type:MS quantitative analysis
Project Summary:Multidrug-resistant bacteria present a major threat to public health. Therefore, new drugs or approaches are urgently needed to manage and mitigate this threat. Here, we screen the molecular candidates that allow the survival of mice upon multidrug-resistant Vibrio parahaemolyticus infection by integrated proteomic and metabolomics analysis, where L-Alanine metabolism and phagocytosis are highly correlated. The role of L-Alanine on boosting mouse survival is further confirmed with in vivo bacterial challenge studies on multidrug-resistant bacteria including V. parahaemolyticus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae. Functional studies demonstrate that exogenous L-Alanine promotes phagocytosis to these different species of multidrug-resistant pathogens. The underlying mechanism involves two events that are L-Alanine-dependently increased TLR4 expression, and L-Alanine-enhanced TLR4 signaling via increasing the biosynthesis and secretion of fatty acids such as palmitate. Palmitate enhances the binding of LPS to TLR4 and thereby promotes TLR4 dimmer formation and endocytosis for the subsequent activation of PI3K/Akt and NF-κB pathways and phagocytosis of bacteria. These results suggest that modulation of metabolic environment is a plausible approach for combating infection with multidrug-resistant bacteria.
Institute:sun yat-sen university
Last Name:jiang
First Name:ming
Address:No. 135, Xingang Xi Road, Guangzhou, 510275, P. R. China, guangzhou, guangdong, 510006, China
Email:jiangm28@mail.sysu.edu.cn
Phone:13434283781

Subject:

Subject ID:SU003031
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA31666620120522-zhxl-test-S1-1control
SA31666720120522-zhxl-test-S3-1control
SA31666820120522-zhxl-test-S3-2control
SA31666920120522-zhxl-test-S2-2control
SA31667020120522-zhxl-test-S1-2control
SA31667120120522-zhxl-test-S2-1control
SA31667220120522-zhxl-C9-2infection
SA31667320120522-zhxl-C10-1infection
SA31667420120522-zhxl-C8-2infection
SA31667520120522-zhxl-C10-2infection
SA31667620120522-zhxl-C9-1infection
SA31667720120522-zhxl-A2-L-1infection
SA31667820120522-zhxl-A2-L-2infection
SA31667920120522-zhxl-C8-1infection
SA31668020120522-zhxl-A1-L-2infection
SA31668120120522-zhxl-A1-L-1infection
SA31668220120522-zhxl-C6-1infection
SA31668320120522-zhxl-C4-2infection
SA31668420120522-zhxl-C4-1infection
SA31668520120522-zhxl-C3-2infection
SA31668620120522-zhxl-C5-1infection
SA31668720120522-zhxl-C5-2infection
SA31668820120522-zhxl-C7-1infection
SA31668920120522-zhxl-C6-2infection
SA31669020120522-zhxl-A3-L-1infection
SA31669120120522-zhxl-C7-2infection
SA31669220120522-zhxl-A5-L-1infection
SA31669320120522-zhxl-C2-L-1infection
SA31669420120522-zhxl-C1-L-2infection
SA31669520120522-zhxl-C1-L-1infection
SA31669620120522-zhxl-B3-L-2infection
SA31669720120522-zhxl-C2-L-2infection
SA31669820120522-zhxl-C3-L-1infection
SA31669920120522-zhxl-C4-L-2infection
SA31670020120522-zhxl-C4-L-1infection
SA31670120120522-zhxl-C3-L-2infection
SA31670220120522-zhxl-B3-L-1infection
SA31670320120522-zhxl-B2-L-2infection
SA31670420120522-zhxl-A5-L-2infection
SA31670520120522-zhxl-C3-1infection
SA31670620120522-zhxl-A4-L-2infection
SA31670720120522-zhxl-A4-L-1infection
SA31670820120522-zhxl-A6-L-1infection
SA31670920120522-zhxl-A6-L-2infection
SA31671020120522-zhxl-B2-L-1infection
SA31671120120522-zhxl-B1-L-2infection
SA31671220120522-zhxl-B1-L-1infection
SA31671320120522-zhxl-A3-L-2infection
SA31671420120522-zhxl-C1-1infection
SA31671520120522-zhxl-A8-1infection
SA31671620120522-zhxl-A7-2infection
SA31671720120522-zhxl-A7-1infection
SA31671820120522-zhxl-A6-2infection
SA31671920120522-zhxl-A8-2infection
SA31672020120522-zhxl-A9-1infection
SA31672120120522-zhxl-A10-2infection
SA31672220120522-zhxl-A10-1infection
SA31672320120522-zhxl-A9-2infection
SA31672420120522-zhxl-A6-1infection
SA31672520120522-zhxl-A5-2infection
SA31672620120522-zhxl-A2-2infection
SA31672720120522-zhxl-A2-1infection
SA31672820120522-zhxl-A1-2infection
SA31672920120522-zhxl-A1-1infection
SA31673020120522-zhxl-A3-1infection
SA31673120120522-zhxl-A3-2infection
SA31673220120522-zhxl-A5-1infection
SA31673320120522-zhxl-A4-2infection
SA31673420120522-zhxl-A4-1infection
SA31673520120522-zhxl-B1-1infection
SA31673620120522-zhxl-B1-2infection
SA31673720120522-zhxl-B9-1infection
SA31673820120522-zhxl-B8-2infection
SA31673920120522-zhxl-B8-1infection
SA31674020120522-zhxl-B7-2infection
SA31674120120522-zhxl-B9-2infection
SA31674220120522-zhxl-B10-1infection
SA31674320120522-zhxl-C2-1infection
SA31674420120522-zhxl-C1-2infection
SA31674520120522-zhxl-B10-2infection
SA31674620120522-zhxl-B7-1infection
SA31674720120522-zhxl-B6-2infection
SA31674820120522-zhxl-B3-2infection
SA31674920120522-zhxl-B3-1infection
SA31675020120522-zhxl-B2-2infection
SA31675120120522-zhxl-B2-1infection
SA31675220120522-zhxl-B4-1infection
SA31675320120522-zhxl-B4-2infection
SA31675420120522-zhxl-B6-1infection
SA31675520120522-zhxl-B5-2infection
SA31675620120522-zhxl-B5-1infection
SA31675720120522-zhxl-C2-2infection
Showing results 1 to 92 of 92

Collection:

Collection ID:CO003024
Collection Summary:Samples were counted, washed with cold PBS and then flash-frozen in liquid N2
Sample Type:Spleen

Treatment:

Treatment ID:TR003040
Treatment Summary:Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample.

Sample Preparation:

Sampleprep ID:SP003037
Sampleprep Summary:Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample.

Combined analysis:

Analysis ID AN004788
Analysis type MS
Chromatography type GC
Chromatography system Thermo Scientific Trace GC Ultra with DSQ II GC/MS
Column Agilent DB5-MS (30m x 0.25mm, 0.25um)
MS Type EI
MS instrument type Triple quadrupole
MS instrument name Thermo Scientific Trace GC Ultra with DSQ II GC/MS
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH003619
Chromatography Summary:Low pH polar (LC/MS Pos early)
Instrument Name:Thermo Scientific Trace GC Ultra with DSQ II GC/MS
Column Name:Agilent DB5-MS (30m x 0.25mm, 0.25um)
Column Temperature:270 °C
Flow Gradient:none
Flow Rate:1.0 mL/min
Solvent A:none
Solvent B:none
Chromatography Type:GC

MS:

MS ID:MS004534
Analysis ID:AN004788
Instrument Name:Thermo Scientific Trace GC Ultra with DSQ II GC/MS
Instrument Type:Triple quadrupole
MS Type:EI
MS Comments:samples was derivatized and then used to firstly protect carbonyl moieties through methoximation, through a 90 min 37 ℃ reaction with 40 μL of 20 mg/mL methoxyamine hydrochloride (Sigma-Aldrich) in pyridine, followed by derivatization of acidic protons through a 30 min 37 0C reaction with the addition of 80 μL N-methyl-N-trimethylsilyltrifluoroace-tamide (MSTFA, Sigma-Aldrich). The derivatized sample of 1 μL was injected into a 30m × 250 μm i.d. × 0.25 μm DBS-MS column using splitless injection and analysis was carried out by Trace DSQ II (Thermo Scientific). The initial temperature of the GC oven was held at 85 0C for 5 min followed by an increase to 330 0C at a rate of 15 0C min-1 then held for 5 min. Helium was used as carrier gas and flow was kept constant at 1 mL min-1. The MS was operated in a range of 50-600 m/z.
Ion Mode:POSITIVE
  logo