Summary of study ST000095

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000087. The data can be accessed directly via it's Project DOI: 10.21228/M8F30Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000095
Study TitleDysfunctional lipid metabolism underlies the effect of perinatal DDT exposure on the development of metabolic syndrome
Study TypeChemical dosage and feeding study
Study SummaryTargeted metabolomic analysis of bile acids was performed on 15 mouse liver samples collected from mice euthanized at 9 months following consumption of a high fat diet w/o perinatal DDT exposure. Funded by the National Institute of Health (R00 ES019919, R03 DK082724, U24 DK092993, U24 DK097154, T32 ES007059, and P60 DK020541), the American Diabetes Association, and USDA-ARS intramural Project 5306-51530-019-00D. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization.
Institute
University of California, Davis
DepartmentU.S.D.A. Western Human Nutrition Research Center
LaboratoryNewman
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Emailjohn.newman@ars.usda.gov
Phone+1-530-752-1009
Submit Date2014-07-10
Num Groups2
Total Subjects15
Raw Data AvailableYes
Raw Data File Type(s)mzML
Uploaded File Size23 M
Analysis Type DetailLC-MS
Release Date2015-02-03
Release Version1
John Newman John Newman
https://dx.doi.org/10.21228/M8F30Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000087
Project DOI:doi: 10.21228/M8F30Z
Project Title:Dysfunctional lipid metabolism underlies the effect of perinatal DDT exposure on the development of metabolic syndrome
Project Type:Animal chemical exposure and feeding study
Project Summary:This study evaluated the effect of perinatal DDT exposure on metabolic syndrome in mice exposed to doses that mimicked human exposure. The samples tested were from 2 groups: 1) a group with perinatal exposure to DDT consuming a high fat diet (DDHF), 2) a control group consuming a high fat diet (CHF). Results indicated the DDHF group had comparatively elevated plasma concentrations of glucose, insulin, cholesterol, and triglycerides, CYP7A1 gene expression, homeostasis model assessment- insulin resistance (HOMA-IR), and fat mass (%), as well as reduced thermogenesis. These data suggest perinatal DDT exposure may cause the co-occurrence of conditions associated with metabolic syndrome, including glucose intolerance, hyperinsulinemia, and dyslipidemia, impaired thermogenesis, and obesity.
Institute:University of California, Davis
Department:Environmental Toxicology
Laboratory:La Merrill
Last Name:La Merrill
First Name:Michele
Address:1 Shields Ave, Davis CA
Email:mlamerrill@ucdavis.edu
Phone:+1-530-754-7254
Funding Source:National Institute of Health (R00 ES019919, R03 DK082724, U24 DK092993, U24 DK097154, T32 ES007059, and P60 DK020541), the American Diabetes Association, and USDA-ARS intramural Project 5306-51530-019-00D

Subject:

Subject ID:SU000114
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:9 months
Gender:Female
Animal Housing:Singly housed
Animal Feed:High fat diet (HFD, 4.73 kcal/g, 20% protein, 35% carbohydrate, and 45% fat per kcal; D12451, Research Diets, New Brunswick, NJ)
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA005396T43-1DDT
SA005397T26-6DDT
SA005398T31-6DDT
SA005399T52-6DDT
SA005400T36-4DDT
SA005401T12-4DDT
SA005402T30-2DDT
SA005403T18-3DDT
SA005404V78-6OIL
SA005405V38-9OIL
SA005406V47-6OIL
SA005407V77-5OIL
SA005408V44-2OIL
SA005409V69-3OIL
SA005410V32-6OIL
Showing results 1 to 15 of 15

Collection:

Collection ID:CO000097
Collection Protocol Filename:Newman_Lab_Bile_Acids_Extraction_&_Analysis_Protocol_La_Merrill_WCMC_P&F.pdf
Sample Type:Liver tissue
Collection Method:Dissection
Storage Conditions:- 80°C
Storage Vials:Eppendorf tubes

Treatment:

Treatment ID:TR000115
Treatment Summary:1.7 mg DDT/kg body weight was administered to dams daily on gestational day 11 through postnatal day 5 and litters were culled to 6 mice at postnatal day 6. Mice were metabolically screened from 2-6 months when they consumed a high fat diet. Mice were sacrificed at 9 months of age.
Treatment Protocol Filename:Newman_Lab_Bile_Acid_Protocols_La_Merrill_WCMC_P&F.pdf
Treatment:Intervention
Treatment Compound:p,p`-DDT, o,p`-DDT
Treatment Route:Oral
Treatment Dose:77.2% p,p`-DDT (98.5% purity neat, AccuStandard, New Haven, CT) and 22.8% o,p`-DDT (100% purity neat, AccuStandard) were dissolved at a final concentration of 0.17 g DDT mixture/L organic olive oil
Treatment Doseduration:From 11.5 days post coitus to postnatal day 5
Treatment Vehicle:Olive oil
Animal Endp Euthanasia:9 months
Animal Endp Tissue Coll List:Liver
Animal Endp Tissue Proc Method:Dissected, placed on dry ice

Sample Preparation:

Sampleprep ID:SP000110
Sampleprep Summary:A 15 mg liver sample (n=1 female/litter in 7 VEH and 8 DDT litters) was pulverized on dry ice, enriched with deuterated bile acid surrogates, butylated hydroxytoluene and ethylinediaminetetraacetic acid, and extracted twice with 500 uL methanol. The combined extract was dried, reconstituted in 100uL 50:50 methanol:acetonitrile with internal standards 1-phenyl-3-hexanoic acid urea and 1-cyclohexyl-3-dodecanoic acid urea (Sigma-Aldrich, St. Louis, MO) and filtered at 0.1 µm.
Sampleprep Protocol Filename:Newman_Lab_Bile_Acid_Protocols_La_Merrill_WCMC_P&F.pdf
Processing Method:Pulverization on dry ice
Extraction Method:Methanol
Extract Concentration Dilution:Dried with Rotoevaporator
Extract Storage:Placed in 10 °C autosampler and immediately analyzed
Sample Spiking:Spiked with deuterated bile acid surrogates.
Organ:Liver

Combined analysis:

Analysis ID AN000151
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity UPLC
Column Waters Acquity BEH C18 (100 x 2mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex API 6500 QTrap
Ion Mode NEGATIVE
Units % abundance

Chromatography:

Chromatography ID:CH000107
Chromatography Summary:Targeted UPLC-MS/MS method
Methods Filename:Newman_Lab_Bile_Acids_Extraction_&_Analysis_Protocol_La_Merrill_WCMC_P&F.pdf
Instrument Name:Waters Acquity UPLC
Column Name:Waters Acquity BEH C18 (100 x 2mm, 1.7um)
Column Temperature:60 C
Flow Gradient:Yes
Flow Rate:0.4mL/min
Internal Standard:See protocol/methods file
Sample Injection:5 L
Solvent A:0.1% formic acid
Solvent B:0.1% formic acid in acetonitrile
Analytical Time:16 min
Weak Wash Solvent Name:20% methanol, 10% isopropanol
Weak Wash Volume:600 L
Strong Wash Solvent Name:50:50 Acetonitrile:Methanol
Strong Wash Volume:600 L
Sample Loop Size:17 L
Chromatography Type:Reversed phase

MS:

MS ID:MS000127
Analysis ID:AN000151
Instrument Name:ABI Sciex API 6500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Ion Source Temperature:See protocol/methods file
Ion Spray Voltage:See protocol/methods file
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