Summary of study ST000278

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000222. The data can be accessed directly via it's Project DOI: 10.21228/M8TP4R This work is supported by NIH grant, U2C- DK119886.

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Study IDST000278
Study TitleViral Effect on Metabolism (part I)
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study SummarySmall airway epithelial cell (SAEC) (Lonza Walkersville, Inc., MD) were grown according to the manufacturer's instructions in growth medium (SAGM) containing 0.03 mg/ml bovine pituitary extract (BPE), 0.5 µg/ml hydrocortisone, 0.5 ng/ml hEGF, 0.5 µg/ml epinephrine, 10 µg/ml transferrin, 5 µg/ml insulin, 0.1 ng/ml retinoic acid, 6.5ng/ml triiodothyronine, 50 µg/ml gentamicin and 50ng/ml Amphotericin-B, and 0.5 mg/ml bovine serum albumin (BSA, fatty acid free). When SAE grew to around 80 to 90% confluence, the cells were put into basal medium not supplemented with growth factors 3hours. Then the cell monolayers were infected with naïve hMPV at multiplicity of infection (MOI) of 3 to certain time. The supernatant was saved and cells were harvested and stored exactly according to the protocol provided by University of Michigan Metabolomics Core Facility for metabolomics analysis. 50mM ammonium acetate in water was used for rinse buffer.
Institute
University of Michigan
DepartmentDepartment of Pediatrics
LaboratoryCasola Lab (University of Texas Medical Branch)
Last NameZhao
First NameYanhua
AddressGalveston, TX
Emailyazhao1@utmb.edu
Phone409-443-1461
Submit Date2014-10-24
Num Groups4
Total Subjects12
Analysis Type DetailGC/LC-MS
Release Date2016-01-13
Release Version1
Yanhua Zhao Yanhua Zhao
https://dx.doi.org/10.21228/M8TP4R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000222
Project DOI:doi: 10.21228/M8TP4R
Project Title:Viral Effect on Metabolism
Project Type:Glycolysis/TCA/Nucleotide analysis (tissue/cells)
Project Summary:Global Metabolic profiling of infection by respiratory virus
Institute:University of Texas
Department:Department of Pediatrics
Laboratory:Casola Lab
Last Name:Casola
First Name:Antonella
Address:Galveston, TX
Email:yazhao1@utmb.edu
Phone:409-772-2798

Subject:

Subject ID:SU000298
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id INFECTION TIME (0-24 Hrs)
SA012243S000166640
SA012244S000166660
SA012245S000166650
SA012249S0001667115
SA012250S0001667215
SA012251S0001667015
SA012252S0001667524
SA012253S0001667324
SA012254S0001667424
SA012246S000166686
SA012247S000166696
SA012248S000166676
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000292
Sample Type:Cell Line

Treatment:

Treatment ID:TR000312

Sample Preparation:

Sampleprep ID:SP000306
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000443 AN000444
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6530 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units pmol/µg protein pmol/µg protein

Chromatography:

Chromatography ID:CH000314
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001308
Methods ID:AQM011
Methods Filename:ALPHA_KETO_ACIDS-FULL.M.zip
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000384
Analysis ID:AN000443
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000385
Analysis ID:AN000444
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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