Summary of study ST001179

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000791. The data can be accessed directly via it's Project DOI: 10.21228/M8F97P This work is supported by NIH grant, U2C- DK119886.

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Study IDST001179
Study TitleMetabolomic analysis of skeletal muscle in young and aged mice
Study SummarySarcopenia is the age-induced, progressive loss of skeletal muscle mass and function, which results in poor muscle performance. To better understand changes in skeletal muscles during sarcopenia, we performed a metabolomic analysis of skeletal muscle in young (8-week-old) and aged (28-month-old) mice using CE-TOFMS. Our data shows that the metabolites including glucose and polyamine metabolism were decreased in aged mice compared with young mice. In addition, neurotransmitter levels were higher in aged mice.
Institute
Kyoto Prefectural University
Last NameKamei
First NameYasutomi
Address1-5 Hangi-cho, Shimogamo, Sakyo-ku Kyoto, Kyoto, Kyoto, 606-8522, Japan
Emailran960116@yahoo.co.jp
Phone+81-75-703-5661
Submit Date2019-05-06
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Yasutomi Kamei Yasutomi Kamei
https://dx.doi.org/10.21228/M8F97P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000791
Project DOI:doi: 10.21228/M8F97P
Project Title:Metabolomic analysis of skeletal muscle in young and aged mice
Project Summary:To better understand the metabolic changes in skeletal muscle during aging, we performed a metabolomic analysis of skeletal muscle in young (8-week-old) and aged (28-month-old) mice using CE-TOFMS.
Institute:Kyoto Prefectural University
Last Name:Kamei
First Name:Yasutomi
Address:1-5 Hangi-cho, Shimogamo, Sakyo-ku Kyoto, Kyoto, Kyoto, 606-8522, Japan
Email:ran960116@yahoo.co.jp
Phone:+81-75-703-5661

Subject:

Subject ID:SU001245
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:wild type
Age Or Age Range:8-week-old and 28-month-old
Weight Or Weight Range:25.4 ± 0.2 g for young and 32.0 ± 0.2 g for aged
Gender:Male
Animal Animal Supplier:Shimizu Laboratory Supplies (Kyoto, Japan)
Animal Light Cycle:12-h light-dark cycle
Animal Feed:CE2
Species Group:C57BL/6J

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Age
SA081606Old-328m
SA081607Old-428m
SA081608Old-528m
SA081609Old-228m
SA081610Old-128m
SA081611Young-28w
SA081612Young-38w
SA081613Young-48w
SA081614Young-58w
SA081615Young-18w
Showing results 1 to 10 of 10

Collection:

Collection ID:CO001239
Collection Summary:Gastrocnemius muscles were collected and flash-frozen in liquid N2.
Sample Type:Muscle
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001260
Treatment Summary:No specific treatment to mention.

Sample Preparation:

Sampleprep ID:SP001253
Sampleprep Summary:Gastrocnemius muscles of 8-week-old male mice (N=5, young group) and 28-month-old male mice (N=5, aged group) were used for the metabolomic analysis (Human Metabolome Technologies Inc., Tsuruoka, Japan). Frozen muscle specimens were transferred into 500 μL of methanol containing 50 mM external standard. After homogenization by BMSM10N21 (BMS, Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200 μL of ultrapure water were added to the homogenate. The solution was mixed well and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then dissolved in 50 μL of ultrapure water. The samples obtained were then subjected to CE-TOFMS analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara, CA) at 4 °C. The detected peaks were aligned according to their m/z values and normalized migration times. The peaks were mean-centered and scaled using their standard deviations on a per peak basis as a pretreatment.

Combined analysis:

Analysis ID AN001956 AN001957
Analysis type MS MS
Chromatography type None (Direct infusion) None (Direct infusion)
Chromatography system none none
Column none none
MS Type ESI ESI
MS instrument type Other Other
MS instrument name Agilent CE-TOFMS Agilent CE-TOFMS
Ion Mode POSITIVE NEGATIVE
Units fold fold

Chromatography:

Chromatography ID:CH001418
Instrument Name:none
Column Name:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS001811
Analysis ID:AN001956
Instrument Name:Agilent CE-TOFMS
Instrument Type:Other
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS001812
Analysis ID:AN001957
Instrument Name:Agilent CE-TOFMS
Instrument Type:Other
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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