Summary of study ST001324

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000901. The data can be accessed directly via it's Project DOI: 10.21228/M8770G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001324
Study TitleMetabolomics Adaptation of Juvenile Pacific Abalone Haliotis discus hannai to Heat Stress
Study SummaryWe compared two groups of abalones (from the same population) with different temperature acclimation history, through their survival at acute heat treatment and metabolites changes. The results indicated significantly higher survival for the high temperature acclimation group. Both groups experienced mitochondrial homeostasis break down during heat treatment, while the higher temperature acclimation group accumulated more metabolites beneficial to metabolic homeostasis, including various dipeptides, antioxidants, and neuroprotective substances.
Institute
Institute of Oceanology, Chinese Academy of Sciences
Last NameXu
First NameFei
Address7 Nanhai Road, Qingdao, Shandong, 266071, China
Emailxufei@qdio.ac.cn
Phone+86-13791940813
Submit Date2020-01-30
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2020-09-10
Release Version1
Fei Xu Fei Xu
https://dx.doi.org/10.21228/M8770G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000901
Project DOI:doi: 10.21228/M8770G
Project Title:Pacific abalone metabonomics: exposure to environmental heat stress
Project Type:LC-MS quantitative analysis
Project Summary:Group comparative nontarget-metabolomics study on samples from digestive gland of Pacific abalone
Institute:Institute of Oceanology, Chinese Academy of Sciences
Last Name:Xu
First Name:Fei
Address:7 Nanhai Road, Qingdao, Shandong, 266071, China
Email:xufei@qdio.ac.cn
Phone:+86-13791940813

Subject:

Subject ID:SU001398
Subject Type:Invertebrate
Subject Species:Haliotis discus hannai
Taxonomy ID:42344
Weight Or Weight Range:2.17 ± 0.56 g

Factors:

Subject type: Invertebrate; Subject species: Haliotis discus hannai (Factor headings shown in green)

mb_sample_id local_sample_id Acclimatization temperature Heat exposure
SA096050L_810°C -
SA096051L_910°C -
SA096052L_710°C -
SA096053L_610°C -
SA096054L_110°C -
SA096055L_310°C -
SA096056L_210°C -
SA096057L_410°C -
SA096058L_510°C -
SA096059H_730°C -
SA096060H_830°C -
SA096061H_130°C -
SA096062H_630°C -
SA096063H_230°C -
SA096064H_930°C -
SA096065H_330°C -
SA096066H_430°C -
SA096067H_530°C -
SA096026QC01- -
SA096027QC05- -
SA096028QC06- -
SA096029QC03- -
SA096030QC02- -
SA096031QC04- -
SA096032HH_4- group H exposure to 31°C for 3hours
SA096033HH_2- group H exposure to 31°C for 3hours
SA096034HH_5- group H exposure to 31°C for 3hours
SA096035HH_3- group H exposure to 31°C for 3hours
SA096036HH_9- group H exposure to 31°C for 3hours
SA096037HH_1- group H exposure to 31°C for 3hours
SA096038HH_8- group H exposure to 31°C for 3hours
SA096039HH_6- group H exposure to 31°C for 3hours
SA096040HH_7- group H exposure to 31°C for 3hours
SA096041LH_3- group L exposure to 31°C for 3hours
SA096042LH_2- group L exposure to 31°C for 3hours
SA096043LH_1- group L exposure to 31°C for 3hours
SA096044LH_4- group L exposure to 31°C for 3hours
SA096045LH_5- group L exposure to 31°C for 3hours
SA096046LH_9- group L exposure to 31°C for 3hours
SA096047LH_7- group L exposure to 31°C for 3hours
SA096048LH_8- group L exposure to 31°C for 3hours
SA096049LH_6- group L exposure to 31°C for 3hours
Showing results 1 to 42 of 42

Collection:

Collection ID:CO001393
Collection Summary:Hepatopancreas tissue was dissected from four groups of juvenile abalones (L, H, LH and HH) on ice, washed by deionized water and drain with a neutral filter.
Sample Type:new

Treatment:

Treatment ID:TR001413
Treatment Summary:Within each group, tissues from three abalones were mixed together and ground into powder in liquid nitrogen for subsequent metabolite extraction and metabonomics-based analysis. In this way, nine biological replications were produced for each group. Samples were stored in liquid nitrogen.

Sample Preparation:

Sampleprep ID:SP001406
Sampleprep Summary:50 mg of sample was taken and placed in an Eppendorf tube, followed by the addition of 1000 µl extraction solvent containing an internal target (V methanol : V acetonitrile : V water = 2 : 2 : 1, containing internal standard 2 µg/ml). The mixture was homogenized in ball mill for 4 min at 45 Hz, then ultrasound treated for 5min (incubated in ice water). After homogenization for 3 times, the mixture was incubated for 1 h at -20 °C to precipitate proteins. Centrifugation was performed at 12000 rpm for 15 min at 4 °C. A total of 825 µl supernatant was carefully transferred into a fresh Eppendorf tube, and dried in a vacuum concentrator without heating. 200 µl extraction solvent (V acetonitrile: V water = 1:1) was then added for reconstitution, followed by vortex 30 s and sonicated for 10 min (4 °C water bath), centrifuge for 15 min at 12000 rpm (4 °C). 75 µl supernatant was transferred into a fresh 2 ml LC/MS glass vial for UHPLC-QTOF-MS analysis, while another 20 µl was taken from each sample and pooled as QC samples. Six technical replicates were conducted to QC sample with 75 µL each for UHPLC-QTOF-MS analysis.
Sampleprep Protocol Filename:LC-MS-Q211.pdf

Combined analysis:

Analysis ID AN002202 AN002203
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1290 Agilent 1290
Column Waters Acquity UPLC BEH Amide (100 x 2.1mm, 1.7µm) Waters Acquity UPLC BEH Amide (100 x 2.1mm, 1.7µm)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name ABI Sciex 5600 TripleTOF ABI Sciex 5600 TripleTOF
Ion Mode POSITIVE NEGATIVE
Units mV*s mV*min

Chromatography:

Chromatography ID:CH001616
Chromatography Summary:UHPLC
Methods Filename:LC-MS-Q211.pdf
Instrument Name:Agilent 1290
Column Name:Waters Acquity UPLC BEH Amide (100 x 2.1mm, 1.7µm)
Flow Gradient:40%-95% B
Flow Rate:500 µL/min
Solvent A:25 mM NH4OAc and 25 mM NH4OH in water (pH = 9.75)
Solvent B:acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS002048
Analysis ID:AN002202
Instrument Name:ABI Sciex 5600 TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:MS raw data files were converted to the mzXML format using ProteoWizard, and processed by R package XCMS (version 3.2). The preprocessing results generated a data matrix that consisted of the retention time, mass-to-charge ratio (m/z) values, and peak intensity. R package CAMERA was used for peak annotation after XCMS data processing.
Ion Mode:POSITIVE
  
MS ID:MS002049
Analysis ID:AN002203
Instrument Name:ABI Sciex 5600 TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:MS raw data files were converted to the mzXML format using ProteoWizard, and processed by R package XCMS (version 3.2). The preprocessing results generated a data matrix that consisted of the retention time, mass-to-charge ratio (m/z) values, and peak intensity. R package CAMERA was used for peak annotation after XCMS data processing.
Ion Mode:NEGATIVE
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