Summary of study ST001448

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000995. The data can be accessed directly via it's Project DOI: 10.21228/M8311J This work is supported by NIH grant, U2C- DK119886.

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Study IDST001448
Study TitleMaternal Hypercortisolemia alters placental metabolism: NMR metabolomics
Study SummaryNMR metabolomic studies of placental tissue from sheep with excess maternal cortisol during late gestation
Institute
University of Florida
Last NameWalejko
First NameJacquelyn
Address300 N Duke St Durham NC 27701
Emailjacquelyn.walejko@duke.edu
Phone9194792304
Submit Date2020-08-18
Raw Data AvailableYes
Raw Data File Type(s)pdata, scon2, .par,
Analysis Type DetailNMR
Release Date2020-09-16
Release Version1
Jacquelyn Walejko Jacquelyn Walejko
https://dx.doi.org/10.21228/M8311J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000995
Project DOI:doi: 10.21228/M8311J
Project Title:Maternal Hypercortisolemia alters placental metabolism
Project Summary:Previous studies have suggested that increases in maternal cortisol or maternal stress in late pregnancy increase the risk of stillbirth at term. In an ovine model with increased maternal cortisol over the last 0.20 of gestation, we have previously found evidence of disruption of fetal serum and cardiac metabolomics, and altered expression of genes related to mitochondrial function and metabolism in biceps femoris, diaphragm and cardiac muscle. The present studies were designed to test for effects of chronically increased maternal cortisol on gene expression and metabolomics in placentomes near term. We hypothesized that changes in placenta may underlie or contribute to the alterations in fetal serum metabolomics, and thereby contribute to changes in striated muscle metabolism. Placentomes were collected from pregnancies in early labor (143±1 d gestation) of control ewes (n=7) or ewes treated with cortisol (1 mg/kg/d iv; n=5) starting at day 115 of gestation. Transcriptomics and metabolomics were performed using an ovine gene expression microarray (Agilent 019921) and HR-MAS NMR, respectively. Multi-omic analysis indicates that amino acid metabolism, particularly of branched chain amino acids and glutamate occur in placenta; changes in amino acid metabolism, degradation or biosynthesis in placenta were consistent with changes in valine, isoleucine, leucine and glycine in fetal serum. The analysis also indicates changes in glycerophospholipid metabolism and suggests changes in ER stress and antioxidant status in the placenta. These findings suggest that changes in placental function occurring with excess maternal cortisol in late gestation may contribute to metabolic dysfunction at birth.
Institute:University of Florida
Department:Pharmacodynamics
Last Name:Keller-Wood
First Name:Maureen
Address:1345 SW Archer Rd, PO 100487, Gainesville, FL 32610
Email:kellerwd@cop.ufl.edu
Phone:352-273-7687
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