Summary of study ST001643

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001051. The data can be accessed directly via it's Project DOI: 10.21228/M8V702 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001643
Study TitleDeletion of the diabetes candidate gene Slc16a13 in mice
Study SummaryThe metabolome of plasma and liver lysates of Slc16a13 knockout mice was analyzed. Genome-wide association studies identified SLC16A13 as novel target gene in type 2 diabetes. The SLC16A13 gene encodes for SLC16A13/MCT13, a member of the solute carrier 16 family of monocarboxylate transporters. So far, biology and physiological function of SLC16A13 is unknown. Deletion of Slc16a13 is hypothezised to affect intrahepatocellular monocarboxylate availability, that drives increased oxidative phosphorylation, while reducing hepatic lipid content, thereby attenuating hepatic insulin resistance.
Institute
University of California, Davis
DepartmentWest Coast Metabolomics Center
LaboratoryInstitut für Diabetesforschung und Metabolische Erkrankungen (IDM)
Last NameBirkenfeld
First NameAndreas
AddressOttfried-Müller Str. 10 72076 Tübingen
EmailAndreas.Birkenfeld@med.uni-tuebingen.de
Phone+49(0)7071 84 20257
Submit Date2021-01-06
Raw Data AvailableYes
Raw Data File Type(s).cdf
Analysis Type DetailGC-MS
Release Date2021-04-01
Release Version1
Andreas Birkenfeld Andreas Birkenfeld
https://dx.doi.org/10.21228/M8V702
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001051
Project DOI:doi: 10.21228/M8V702
Project Title:Deletion of the diabetes candidate gene Slc16a13 in mice
Project Summary:The metabolome of plasma and liver lysates of Slc16a13 knockout mice was analyzed. Genome-wide association studies identified SLC16A13 as novel target gene in type 2 diabetes. The SLC16A13 gene encodes for SLC16A13/MCT13, a member of the solute carrier 16 family of monocarboxylate transporters. So far, biology and physiological function of SLC16A13 is unknown. Deletion of Slc16a13 is hypothezised to affect intrahepatocellular monocarboxylate availability, that drives increased oxidative phosphorylation, while reducing hepatic lipid content, thereby attenuating hepatic insulin resistance.
Institute:University of California, Davis
Department:West Coast Metabolomics Center
Last Name:Birkenfeld
First Name:Andreas
Address:Ottfried-Müller Str. 10 72076 Tübingen
Email:Andreas.Birkenfeld@med.uni-tuebingen.de
Phone:+49(0)7071 84 20257

Subject:

Subject ID:SU001720
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA151276P3_003KO
SA151277P2_002KO
SA151278P4_004KO
SA151279P5_005KO
SA151280P6_006KO
SA151281L1_013KO
SA151282P1_001KO
SA151283L5_017KO
SA151284L3_015KO
SA151285L2_014KO
SA151286L6_018KO
SA151287L4_016KO
SA151288P7_007WT
SA151289P12_012WT
SA151290P8_008WT
SA151291P9_009WT
SA151292L7_019WT
SA151293P11_011WT
SA151294P10_010WT
SA151295L11_023WT
SA151296L9_021WT
SA151297L12_024WT
SA151298L8_020WT
SA151299L10_022WT
Showing results 1 to 24 of 24

Collection:

Collection ID:CO001713
Collection Summary:Mice were euthanized and plasma was collected thereafter. Liver was collected and frozen in LN2
Sample Type:Liver

Treatment:

Treatment ID:TR001733
Treatment Summary:mouse liver and plasma, wildtype and KO

Sample Preparation:

Sampleprep ID:SP001726
Sampleprep Summary:Mice were euthanized and plasma was collected thereafter. Liver was collected and frozen in LN2

Combined analysis:

Analysis ID AN002688
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus IV
Column Rtx-5Sil MS column
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus IV TOF
Ion Mode UNSPECIFIED
Units counts per second

Chromatography:

Chromatography ID:CH001982
Chromatography Summary:A 30 m long, 0.25 mm i.d. Rtx-5Sil MS column (0.25 μm 95% dimethyl 5% diphenyl polysiloxane film) with additional 10 m integrated guard column is used (Restek, Bellefonte PA). 99.9999% pure Helium with built-in purifier (Airgas, Radnor PA) is set at constant flow of 1 ml/min. The oven temperature is held constant at 50°C for 1 min and then ramped at 20°C/min to 330°C at which it is held constant for 5 min.
Instrument Name:Leco Pegasus IV
Column Name:Rtx-5Sil MS column
Chromatography Type:GC

MS:

MS ID:MS002487
Analysis ID:AN002688
Instrument Name:Leco Pegasus IV TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:Injector conditions: Agilent 6890 GC is equipped with a Gerstel automatic liner exchange system (ALEX) that includes a multipurpose sample (MPS2) dual rail, and a Gerstel CIS cold injection system (Gerstel, Muehlheim, Germany) with temperature program as follows: 50°C to 275°C final temperature at a rate of 12 °C/s and hold for 3 minutes. Injection volume is 0.5 μl with 10 μl/s injection speed on a splitless injector with purge time of 25 seconds. Liner (Gerstel #011711-010-00) is changed after every 10 samples, (using the Maestro1 Gerstel software vs. 1.1.4.18). Before and after each injection, the 10 μl injection syringe is washed three times with 10 μl ethyl acetate. Gas Chromatography conditions: A 30 m long, 0.25 mm i.d. Rtx-5Sil MS column (0.25 μm 95% dimethyl 5% diphenyl polysiloxane film) with additional 10 m integrated guard column is used (Restek, Bellefonte PA). 99.9999% pure Helium with built-in purifier (Airgas, Radnor PA) is set at constant flow of 1 ml/min. The oven temperature is held constant at 50°C for 1 min and then ramped at 20°C/min to 330°C at which it is held constant for 5 min. Mass spectrometer settings: A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da.
Ion Mode:UNSPECIFIED
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