Summary of Study ST001684

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001083. The data can be accessed directly via it's Project DOI: 10.21228/M8Q98J This work is supported by NIH grant, U2C- DK119886.

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Study IDST001684
Study TitleAn overexpression of lipoprotein lipase leads to an alteration in the skeletal muscle metabolome in transgenic rabbits
Study SummaryThe purpose of the current study was to characterize the skeletal muscle metabolome in the lipoprotein lipase (LPL) transgenic rabbits. The skeletal muscle metabolite profile was analyzed using capillary electrophoresis time-of flight mass spectrometry in the control rabbits and LPL transgenic rabbits (n = 9, each group).
Institute
Saga University
Last NameNishida
First NameYuichiro
AddressDepartment of Preventive Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501
Emailynishida@cc.saga-u.ac.jp
Phone+81 952 34 2287
Submit Date2020-12-03
Analysis Type DetailLC-MS
Release Date2022-01-02
Release Version1
Yuichiro Nishida Yuichiro Nishida
https://dx.doi.org/10.21228/M8Q98J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001083
Project DOI:doi: 10.21228/M8Q98J
Project Title:An overexpression of lipoprotein lipase leads to an alteration in the skeletal muscle metabolome in transgenic rabbits
Institute:Saga University
Last Name:Nishida
First Name:Yuichiro
Email:ynishida@cc.saga-u.ac.jp
Phone:+81 952 34 2287

Subject:

Subject ID:SU001761
Subject Type:Mammal
Subject Species:Oryctolagus cuniculus
Taxonomy ID:9986

Factors:

Subject type: Mammal; Subject species: Oryctolagus cuniculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA1553922284Transgenic
SA1553932285Transgenic
SA1553942311Transgenic
SA1553952316Transgenic
SA1553962258Transgenic
SA1553972312Transgenic
SA1553982314Transgenic
SA1553992313Transgenic
SA1554002315Transgenic
SA1554012262Wild-type
SA1554022287Wild-type
SA1554032289Wild-type
SA1554042260Wild-type
SA1554052317Wild-type
SA1554062318Wild-type
SA1554072319Wild-type
SA1554082320Wild-type
SA1554092322Wild-type
Showing results 1 to 18 of 18

Collection:

Collection ID:CO001754
Collection Summary:Immediately after the scarification, gastrocnemius muscle was dissected. These muscle samples were immediately frozen in liquid nitrogen, and stored at -80 degree until a metabolome analysis.
Sample Type:Muscle

Treatment:

Treatment ID:TR001774
Treatment Summary:At the end of high fat diet feeding for 16 weeks (40 weeks old), after overnight fasting, rabbits were sacrificed between 08:30 and 12:30 by overdose of thiamylal sodium.

Sample Preparation:

Sampleprep ID:SP001767
Sampleprep Summary:The samples were mixed with 1500 µL of 50% acetonitrile in water (v/v) containing internal standards (20 μM) and homogenized by a homogenizer (4500 rpm, 60 sec × 10 times). The supernatant (400 µL) was then filtrated through 5-kDa cut-off filter (ULTRAFREE-MC-PLHCC, Human Metabolome Technologies, Yamagata, Japan) to remove macromolecules. The filtrate was centrifugally concentrated and resuspended in 50 µL of ultrapure water immediately before the measurement.

Combined analysis:

Analysis ID AN002750 AN002751
Analysis type MS MS
Chromatography type Other Other
Chromatography system Agilent CE Agilent CE
Column Agilent Fused silica capillary (800 mm x 50 um) Agilent Fused silica capillary (800 mm x 50 um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent CE-TOFMS Agilent CE-TOFMS
Ion Mode POSITIVE NEGATIVE
Units Relative Area Relative Area

Chromatography:

Chromatography ID:CH002032
Instrument Name:Agilent CE
Column Name:Agilent Fused silica capillary (800 mm x 50 um)
Chromatography Type:Other

MS:

MS ID:MS002547
Analysis ID:AN002750
Instrument Name:Agilent CE-TOFMS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS002548
Analysis ID:AN002751
Instrument Name:Agilent CE-TOFMS
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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