Summary of Study ST001789

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001133. The data can be accessed directly via it's Project DOI: 10.21228/M87X24 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001789
Study TitleAcute metabolomic changes of plasma in response to endurance exercise
Study SummaryThis study compared the metabolomic differences in plasma of young adult volunteers between before (Pre), immediately after the run (Time 0) and 60 minutes after the 90-min run (Time 60).
Institute
University of California, San Diego
DepartmentDepartment of Medicine
LaboratoryThe Naviaux Lab
Last NameNaviaux
First NameRobert
Address214 Dickinson St., Room C-107
Emailrnaviaux@health.ucsd.edu
Phone619-543-2904
Submit Date2021-04-21
Num Groups3
Total Subjects19
Num Males13
Num Females6
Analysis Type DetailLC-MS
Release Date2022-01-06
Release Version1
Robert Naviaux Robert Naviaux
https://dx.doi.org/10.21228/M87X24
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001133
Project DOI:doi: 10.21228/M87X24
Project Title:Metabolomic changes of cerebrospinal fluid in response to endurance exercise
Project Summary:Metabolomics has emerged as a powerful new tool in precision medicine for gaining mechanistic insight into health and disease. The objective of this project was to investigate the metabolomic changes in cerebrospinal fluid (CSF) in response to endurance exercise. Young adult volunteers were enrolled, and the participants completed a monitored outdoor 90-minute run. CSF samples were collected during rest (Pre), and 60 minutes after the run (Post). Broad-spectrum targeted metabolomics was conducted using the liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). This study provided insights into the neuroprotective and mental health benefits of endurance exercise.
Institute:University of California, San Diego School of Medicine
Last Name:Naviaux
First Name:Robert
Address:214 Dickinson St., Room C-107, San Diego, CA, 92103, USA
Email:rnaviaux@health.ucsd.edu
Phone:619-543-2904

Subject:

Subject ID:SU001866
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:25(IQR 22-31)
Gender:Male and female
Human Nutrition:Participants were given a banana (~100g)

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA166508LP26_plasma_B_MPre
SA166509LP25_plasma_B_MPre
SA166510LP28_plasma_B_MPre
SA166511LP24_plasma_B_MPre
SA166512LP31_plasma_B_MPre
SA166513LP11_plasma_B_FPre
SA166514LP32_plasma_B_MPre
SA166515LP22_plasma_B_MPre
SA166516LP29_plasma_B_MPre
SA166517LP27_plasma_B_MPre
SA166518LP23_plasma_B_FPre
SA166519LP20_plasma_B_FPre
SA166520LP21_plasma_B_MPre
SA166521LP15_plasma_B_FPre
SA166522LP30_plasma_B_FPre
SA166523LP19_plasma_B_FPre
SA166524LP16_plasma_B_MPre
SA166525LP18_plasma_B_MPre
SA166526LP14_plasma_B_MPre
SA166527LP26_plasma_R2_MTime 0
SA166528LP24_plasma_R2_MTime 0
SA166529LP27_plasma_R2_MTime 0
SA166530LP25_plasma_R2_MTime 0
SA166531LP29_plasma_R2_MTime 0
SA166532LP22_plasma_R2_MTime 0
SA166533LP32_plasma_R2_MTime 0
SA166534LP31_plasma_R2_MTime 0
SA166535LP28_plasma_R2_MTime 0
SA166536LP21_plasma_R2_MTime 0
SA166537LP19_plasma_R2_FTime 0
SA166538LP15_plasma_R2_FTime 0
SA166539LP18_plasma_R2_MTime 0
SA166540LP20_plasma_R2_FTime 0
SA166541LP11_plasma_R2_FTime 0
SA166542LP16_plasma_R2_MTime 0
SA166543LP23_plasma_R2_FTime 0
SA166544LP14_plasma_R2_MTime 0
SA166545LP30_plasma_R2_FTime 0
SA166546LP27_plasma_R3_MTime 60
SA166547LP24_plasma_R3_MTime 60
SA166548LP25_plasma_R3_MTime 60
SA166549LP26_plasma_R3_MTime 60
SA166550LP29_plasma_R3_MTime 60
SA166551LP22_plasma_R3_MTime 60
SA166552LP32_plasma_R3_MTime 60
SA166553LP31_plasma_R3_MTime 60
SA166554LP28_plasma_R3_MTime 60
SA166555LP30_plasma_R3_FTime 60
SA166556LP19_plasma_R3_FTime 60
SA166557LP15_plasma_R3_FTime 60
SA166558LP11_plasma_R3_FTime 60
SA166559LP20_plasma_R3_FTime 60
SA166560LP23_plasma_R3_FTime 60
SA166561LP18_plasma_R3_MTime 60
SA166562LP16_plasma_R3_MTime 60
SA166563LP14_plasma_R3_MTime 60
SA166564LP21_plasma_R3_MTime 60
Showing results 1 to 57 of 57

Collection:

Collection ID:CO001859
Collection Summary:Measurements and samples were collected on two days separated by 4 weeks. Samples were drawn at the same time on the two experimental days to control for circadian rhythms. A baseline blood sample (Pre) was collected, the cannula was closed with a screw cap and protected with an elastic bandage. Participants were given a banana (~100g) then completed a 90-minute outdoor run at local time 09:00 to 10:30 am. . Immediately after completing the 90-minute run, a time 0 blood sample was collected (Time 0). After 60 minutes of recovery (1130 am local time), a time 60 blood sample was collected (Time 60).
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001879
Treatment Summary:Participants were given a banana (~100g) then completed a 90-minute outdoor run at local time 09:00 to 10:30 am, maintaining an effort of 75%-80% of their heart rate. A baseline blood sample (Pre) was collected before the run. Immediately after completing the 90-minute run, a time 0 blood sample was collected (Time 0). After 60 minutes of recovery (1130 am local time), a time 60 blood sample was collected (Time 60).

Sample Preparation:

Sampleprep ID:SP001872
Sampleprep Summary:Plasma samples (90 µl) were mixed with 5 µl of commercial internal standards and 5 µl of custom-synthesized 13C labeled standards and then incubated for 10 min at room temperature to permit small molecules and vitamins in the internal standards to associate with plasma binding proteins. The metabolites were extracted with 400 µl of cold (-20 °C) methanol: acetonitrile (50:50, v/v), yielding a final concentration of methanol: acetonitrile: water (40:40:20, v/v/v). The samples were then vortexed vigorously, incubated on crushed ice for 10 min, and then centrifuged at 16,000 g for 10 min at 4 °C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80 °C prior to LC-MS/MS.
Processing Storage Conditions:On ice
Extract Storage:-80℃

Combined analysis:

Analysis ID AN002901 AN002902
Analysis type MS MS
Chromatography type HILIC Reversed phase
Chromatography system Shimadzu 20AD Shimadzu 20AD
Column Shodex NH2P-40 2E (250 × 2 mm, 4 µm) Raptor Biphenyl column (150 × 2.1 mm, 2.7 µm)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap
Ion Mode UNSPECIFIED UNSPECIFIED
Units peak area peak area

Chromatography:

Chromatography ID:CH002151
Chromatography Summary:Ten µL of the extract was injected into the column through a CTC PAL autosampler. The samples were separated in HILIC mode using a polymer-based NH2 column (250 × 2 mm, 4 µm) (Asahipak NH2P-40 2E, Shodex, Japan). The optimal LC conditions were as follows: Mobile phase A: 95% H2O with 20 mM (NH4)2CO3 and 5% ACN, pH 9.8. Mobile phase B: 100% ACN. The gradient was: 0 - 3.5 min 95% B, 3.6 - 8 min 85% B, 8.1 - 13 min 75% B, 14 - 30 min 0% B, 31 - 41 min 95% B, 41.1 min stop. The flow rate was 200 µL/min, and the column temperature was held at 25 ºC.
Instrument Name:Shimadzu 20AD
Column Name:Shodex NH2P-40 2E (250 × 2 mm, 4 µm)
Column Pressure:900 psi - 2400 psi
Column Temperature:25
Flow Gradient:Constant
Flow Rate:200 µL/min
Injection Temperature:4
Solvent A:95% H2O with 20 mM (NH4)2CO3 and 5% ACN, pH 9.8
Solvent B:100% ACN
Oven Temperature:25
Sample Loop Size:10
Sample Syringe Size:100
Chromatography Type:HILIC
  
Chromatography ID:CH002152
Chromatography Summary:Ten µL of the extract was injected into the column through a CTC PAL autosampler. The samples were separated in the RP model using a Raptor Biphenyl column (150 × 2.1 mm, 2.7 µm) (Restek, PA). The LC conditions were as follows: Mobile phase A: 90% H2O with 0.1% formic acid and 10% MEOH, pH 4.0. Mobile phase B: MEOH-IPA (50:50, v/v) with 0.1% formic acid. The gradient was: 0 - 2 min 10% B, 2.1 - 4 min 40% B, 4 -12 min, linear ramping up to 100% B, 12 – 18 min 100% B, 19 - 24 min 10% B, 24.1 min stop. The flow rate was 250 µL/min, and the column temperature was controlled at 40 ºC.
Instrument Name:Shimadzu 20AD
Column Name:Raptor Biphenyl column (150 × 2.1 mm, 2.7 µm)
Column Pressure:2800 psi - 4200 psi
Column Temperature:40
Flow Gradient:Constant
Flow Rate:250 µL/min
Injection Temperature:4
Solvent A:90% H2O with 0.1% formic acid and 10% MEOH, pH 4.0
Solvent B:MEOH-IPA (50:50, v/v) with 0.1% formic acid
Oven Temperature:40
Sample Loop Size:10
Sample Syringe Size:100
Chromatography Type:Reversed phase

MS:

MS ID:MS002693
Analysis ID:AN002901
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:The MS/MS detection was performed using electrospray ionization (ESI) and advanced scheduled multiple reaction monitoring (MRM) with dynamic windowing and fast polarity switch. The ESI source conditions were set as follows: electrospray voltage of -4500 V for negative mode and 5500 V for positive mode, source temperature of 500 °C, curtain gas of 30, ion source gas 1, and gas 2 of 35 psi, respectively.Peaks were manually inspected and integrated using Multiquant 3.0.2. The peak area was exported to excel for further analysis.
Ion Mode:UNSPECIFIED
Collision Energy:Compound-dependent
Ion Source Temperature:500
Ion Spray Voltage:-4500 V for negative and 5500 V for positive
Ionization:ESI
Mass Accuracy:0.1 Da
  
MS ID:MS002694
Analysis ID:AN002902
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:The MS/MS detection was performed using electrospray ionization (ESI) and advanced scheduled multiple reaction monitoring (MRM) with dynamic windowing and fast polarity switch. The ESI source conditions were set as follows: electrospray voltage of -4500 V for negative mode and 5500 V for positive mode, source temperature of 500 °C, curtain gas of 30, ion source gas 1, and gas 2 of 35 psi, respectively.Peaks were manually inspected and integrated using Multiquant 3.0.2. The peak area was exported to excel for further analysis.
Ion Mode:UNSPECIFIED
Collision Energy:Compound-dependent
Ion Source Temperature:500
Ion Spray Voltage:-4500 V for negative and 5500 V for positive
Ionization:ESI
Mass Accuracy:0.1 Da
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