Summary of Study ST001905

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001200. The data can be accessed directly via it's Project DOI: 10.21228/M8KX3M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001905
Study TitleMetabolomic profiling of saliva in diabetes patients
Study SummaryWe performed comprehensive profiling of plasma and salivary metabolomes, and investigated multivariate covariations with clinical markers of oral and cardiometabolic health in healthy subjects and type 2 diabetes patients. The key findings highlight the potential utility of salivary metabolites for reflecting cardiometabolic changes, including hyperglycemia and dyslipidemia. Our study shows that analysis of panels of salivary metabolites may become an attractive alternative to blood testing for screening of metabolic disorders.
Institute
Osaka University
Last NameSakanaka
First NameAkito
Address1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
Emailsakanaka@dent.osaka-u.ac.jp
Phone+81668792922
Submit Date2021-08-15
Analysis Type DetailGC-MS
Release Date2022-12-09
Release Version1
Akito Sakanaka Akito Sakanaka
https://dx.doi.org/10.21228/M8KX3M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001200
Project DOI:doi: 10.21228/M8KX3M
Project Title:Study on the correlation between saliva and plasma in glucose intolerance
Project Summary:Metabolomic profiling of plasma and saliva in type 2 diabetes patients was performed.
Institute:Osaka University
Last Name:Sakanaka
First Name:Akito
Address:Yamadaoka 1-8, Suita, Osaka, 565-0871, Japan
Email:sakanaka@dent.osaka-u.ac.jp
Phone:+81668792922

Subject:

Subject ID:SU001983
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Diagnosis
SA176612C09Control
SA176613C10Control
SA176614C08Control
SA176615C28Control
SA176616C26Control
SA176617C17Control
SA176618C23Control
SA176619C25Control
SA176620C03Control
SA176621C18Control
SA176622C24Control
SA176623C04Control
SA176624C14Control
SA176625C12Control
SA176626C05Control
SA176627C11Control
SA176628C06Control
SA176629C19Control
SA176630C29Control
SA176631C07Control
SA176632C16Control
SA176633C20Control
SA176634C30Control
SA176635C13Control
SA176636C22Control
SA176637C01Control
SA176638C27Control
SA176639C02Control
SA176640C15Control
SA176641C21Control
SA176642P14BDiabetes
SA176643P09BDiabetes
SA176644P27BDiabetes
SA176645P31BDiabetes
SA176646P03BDiabetes
SA176647P33BDiabetes
SA176648P04BDiabetes
SA176649P05BDiabetes
SA176650P02BDiabetes
SA176651P21BDiabetes
SA176652P26BDiabetes
SA176653P07BDiabetes
SA176654P12BDiabetes
SA176655P24BDiabetes
SA176656P20BDiabetes
SA176657P23BDiabetes
SA176658P08BDiabetes
SA176659P30BDiabetes
SA176660P29BDiabetes
SA176661P19BDiabetes
SA176662P22BDiabetes
SA176663P10BDiabetes
SA176664P01BDiabetes
SA176665P25BDiabetes
SA176666P17BDiabetes
SA176667P11BDiabetes
SA176668P15BDiabetes
SA176669P16BDiabetes
SA176670P13BDiabetes
SA176671P06BDiabetes
SA176672P32BDiabetes
Showing results 1 to 61 of 61

Collection:

Collection ID:CO001976
Collection Summary:Four calibrated and licensed dentists performed oral examinations, and saliva collection the same day as blood collection. All subjects were asked to refrain from eating, drinking, or brushing for at least 1 h prior to undergoing these procedures. The subject was asked to expectorate unstimulated whole saliva over a 10-min period into a 50-mL tube (Corning, Corning, NY, USA) kept on ice. Following incubation on ice for 15 min, the aqueous layer of each sample was pipetted off, and samples with volumes of at least 1 and 0.1 mL were aliquoted into 2-mL tubes kept at 4℃ in a CubeCooler® as study and quality control (QC) samples, respectively. Subsequently, they were frozen with liquid nitrogen and stored at −80℃ until analysis.
Sample Type:Saliva

Treatment:

Treatment ID:TR001995
Treatment Summary:Systemically healthy controls and T2D patients were recruited.

Sample Preparation:

Sampleprep ID:SP001989
Sampleprep Summary:Saliva samples were thawed at 4℃, then vortexed and centrifuged (4 ℃, 18,000 × g) for 3 min. Next, 0.8 mL of the aqueous layer was pipetted off and weighed, then 0.3 mL of that was transferred into a 2-mL glass vial (Nichiden-Rika Glass, Kobe, Japan) and kept at 4℃ in a CubeCooler®. For extraction, 0.3 mL of deaerated MilliQ water containing ribitol (0.02 mg/mL) as an internal standard was added. After incubation using an Eppendorf thermomixer (25℃, 1000 rpm, 10 min), 1.4 mL of deaerated acetonitrile was added. After incubation (25℃, 1000 rpm, 10 min) and centrifugation (4℃, 1800 × g) for 3 min, 1.6 mL of the supernatant was transferred to a 2-mL tube and dried with a vacuum concentrator (VC-96R; TAITEC, Koshigaya, Japan) for 30 min, then allowed to lyophilize overnight. Derivatization was performed with a methoxyamine hydrochloride solution with pyridine at a concentration of 20 mg/mL, followed by silylation application of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA).

Combined analysis:

Analysis ID AN003102
Analysis type MS
Chromatography type GC
Chromatography system GC-MS/MS-TQ8040
Column InertCap 5MS/NP capillary
MS Type EI
MS instrument type Triple quadrupole
MS instrument name Shimazu TQ8040
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH002289
Instrument Name:GC-MS/MS-TQ8040
Column Name:InertCap 5MS/NP capillary
Chromatography Type:GC

MS:

MS ID:MS002884
Analysis ID:AN003102
Instrument Name:Shimazu TQ8040
Instrument Type:Triple quadrupole
MS Type:EI
MS Comments:GC-MS data were converted into ABF format using an ABF converter, then processed using MS-DIAL (version 3.90) to perform feature detection, spectra deconvolution, metabolite identification, and peak alignment (Tsugawa et al. 2015). Normalization was then performed based on the internal standard (ribitol) as well as the LOWESS algorithm, whereby metabolic feature signal drift with time was independently corrected by fitting a LOWESS curve to the MS signal measured in QCs.
Ion Mode:POSITIVE
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