Summary of Study ST002997
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001867. The data can be accessed directly via it's Project DOI: 10.21228/M8CX3R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002997 |
| Study Title | Metabolomic analysis reveal mechanisms of Shenzao dripping pill against myocardial ischemia |
| Study Type | Metabolomic analysis on serum samples of SZDP against MI |
| Study Summary | This study employed the advanced Ultra-high performance liquid chromatography-quadruple-Exactive Orbitrap mass spectrometry technology to detect biomarkers related to myocardial ischemia in rats. Additionally, it aimed to investigate the effect of the Shenzao dripping pill on rectifying metabolic disorders and preserving the metabolites in dynamic balance during myocardial ischemia, analyze potential metabolic pathways, and elucidate the mechanism of action of SZDP through the lens of metabolomics. |
| Institute | Guangdong Pharmaceutical University |
| Department | Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province |
| Laboratory | Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine |
| Last Name | Kuang |
| First Name | Jiehui |
| Address | Waihuan East Road, 280, Guangzhou, Guangdong, 510006, China |
| kuang_jieh@163.com | |
| Phone | (020)-222-3333 |
| Submit Date | 2023-12-04 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-12-21 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001867 |
| Project DOI: | doi: 10.21228/M8CX3R |
| Project Title: | SZDP Metabolomic studies |
| Project Type: | LC_MS |
| Project Summary: | A metabolomics study was conducted to elucidate the metabolic mechanism of Shenzao dripping pill against myocardial ischemia. |
| Institute: | Guangdong Pharmaceutical University |
| Department: | Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province |
| Laboratory: | Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine |
| Last Name: | Kuang |
| First Name: | Jiehui |
| Address: | Waihuan East Road, 280, Guangzhou, Guangdong, 510006, China |
| Email: | kuang_jieh@163.com |
| Phone: | (020)-222-3333 |
| Funding Source: | This research was funded by the Project of the Science and Technology Plan Project of Guangzhou (No. 201803010115), the National Natural Science Foundation of China (No. 82173972), and the National Major |
Subject:
| Subject ID: | SU003110 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA326407 | M7 | Coronary ligation surgery |
| SA326408 | M8 | Coronary ligation surgery |
| SA326409 | M6 | Coronary ligation surgery |
| SA326410 | M1 | Coronary ligation surgery |
| SA326411 | M9 | Coronary ligation surgery |
| SA326412 | M2 | Coronary ligation surgery |
| SA326413 | M5 | Coronary ligation surgery |
| SA326414 | M3 | Coronary ligation surgery |
| SA326415 | M4 | Coronary ligation surgery |
| SA326424 | S4 | Sham |
| SA326425 | S2 | Sham |
| SA326426 | S1 | Sham |
| SA326427 | S3 | Sham |
| SA326428 | S5 | Sham |
| SA326429 | S7 | Sham |
| SA326430 | S6 | Sham |
| SA326431 | S8 | Sham |
| SA326416 | SZ4 | SZDP-H |
| SA326417 | SZ1 | SZDP-H |
| SA326418 | SZ3 | SZDP-H |
| SA326419 | SZ5 | SZDP-H |
| SA326420 | SZ7 | SZDP-H |
| SA326421 | SZ2 | SZDP-H |
| SA326422 | SZ8 | SZDP-H |
| SA326423 | SZ6 | SZDP-H |
| Showing results 1 to 25 of 25 |
Collection:
| Collection ID: | CO003103 |
| Collection Summary: | Blood was collected from abdominal aorta, and the serum samples were obtained by separating the supernatant from the blood centrifugation with 3,500 rpm for 10 min after 30 min of coagulation and stored in the refrigerator at −80°C immediately. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR003119 |
| Treatment Summary: | Rats in the model and sham groups were given a solution of 0.5% CMC-Na; in the high dose of the SZDP group was administered 500 mg/kg SZDP. All rats were subjected to intragastric administration once daily. |
Sample Preparation:
| Sampleprep ID: | SP003116 |
| Sampleprep Summary: | In each 200 μL aliquot of serum sample, 600 μL of acetonitrile was added and the mixture was thoroughly mixed for a duration of 1 minute. Then, the mixture was subjected to centrifugation at 13000 rpm and 4 °C for a period of 15 min. The supernatant was collected and evaporated to dryness with nitrogen. Methanol (100 μL) was added, and the reconstituted mixture was vortexed and centrifuged at 13000 rpm and a temperature of 4 °C for 15 min. |
Combined analysis:
| Analysis ID | AN004922 | AN004923 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH003716 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 °C |
| Flow Gradient: | 0–2 minutes, 5% B; 2–5 minutes, 5%–50% B; 5–6 minutes, 50% B; 6–18 minutes, 50%–100% B. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004665 |
| Analysis ID: | AN004922 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry was set to positive and negative ion switching mode using an electrospray ionization source operated at 350 °C. The specific MS conditions were established: the sheath gas flow rate was 55 arb. Units; the auxiliary gas flow rate was 15 arb. Units with a temperature of 200 °C; the electrospray voltage was 3.5 kV in positive ion mode and -3.8 kV in negative ion mode. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004666 |
| Analysis ID: | AN004923 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry was set to positive and negative ion switching mode using an electrospray ionization source operated at 350 °C. The specific MS conditions were established: the sheath gas flow rate was 55 arb. Units; the auxiliary gas flow rate was 15 arb. Units with a temperature of 200 °C; the electrospray voltage was 3.5 kV in positive ion mode and -3.8 kV in negative ion mode. |
| Ion Mode: | NEGATIVE |
