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MGP003281

UniProt Annotations

Entry Information
Gene Nameubiquitin specific peptidase 1
Protein EntryUBP1_HUMAN
UniProt IDO94782
SpeciesHuman
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=0.7 uM for ubiquitin vinyl sulfone (in presence of WDR48) {ECO:0000269|PubMed:18082604}; KM=1.4 uM for ubiquitin vinyl sulfone (in absence of WDR48) {ECO:0000269|PubMed:18082604};
Catalytic ActivityThiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C- terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). {ECO:0000269|PubMed:18082604}.
Developmental StageCell cycle-regulated. Highest level during S phase. {ECO:0000269|PubMed:15694335}.
FunctionNegative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. {ECO:0000269|PubMed:15694335, ECO:0000269|PubMed:16531995, ECO:0000269|PubMed:18082604}.
InductionDown-regulated following DNA damage. {ECO:0000269|PubMed:18082604}.
MiscellaneousHEK293T cells expressing reduced levels of USP1 show a higher level of ubiquitinated PCNA and an increase in point mutations upon UV irradiation.
PtmAutocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis. {ECO:0000269|PubMed:15694335, ECO:0000269|PubMed:16531995}.
PtmUbiquitinated; leading to its subsequent proteasomal degradation. {ECO:0000250}.
Sequence CautionSequence=AAH18745.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305}; Sequence=AAH32364.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305}; Sequence=BAD92130.1; Type=Frameshift; Positions=230; Evidence={ECO:0000305};
SimilarityBelongs to the peptidase C19 family. {ECO:0000305}.
SimilarityContains 1 USP domain. {ECO:0000305}.
Subcellular LocationNucleus {ECO:0000269|PubMed:15694335}.
SubunitInteracts with FANCD2 and PCNA. Interacts with WDR48. {ECO:0000269|PubMed:15694335, ECO:0000269|PubMed:18082604}.
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