MGP Database

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MGP004545

UniProt Annotations

Entry Information
Gene Nameactivating transcription factor 6
Protein EntryATF6A_HUMAN
UniProt IDP18850
SpeciesHuman
Comments
Comment typeDescription
DomainThe basic domain functions as a nuclear localization signal.
DomainThe basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
FunctionTranscription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. {ECO:0000269|PubMed:11158310, ECO:0000269|PubMed:11779464}.
InteractionQ68CJ9:CREB3L3; NbExp=2; IntAct=EBI-852157, EBI-852194;
PtmDuring unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. {ECO:0000269|PubMed:11163209}.
PtmN-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR). {ECO:0000269|PubMed:14699159}.
PtmPhosphorylated in vitro by MAPK14/P38MAPK.
SimilarityBelongs to the bZIP family. ATF subfamily. {ECO:0000305}.
SimilarityContains 1 bZIP (basic-leucine zipper) domain. {ECO:0000255|PROSITE-ProRule:PRU00978}.
Subcellular LocationEndoplasmic reticulum membrane; Single-pass type II membrane protein.
Subcellular LocationProcessed cyclic AMP-dependent transcription factor ATF-6 alpha: Nucleus. Note=Under ER stress the cleaved N- terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling.
SubunitHomodimer and heterodimer with ATF6-beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). Interacts also with the transcription factors GTF2I, YY1 and SRF. Interacts (via lumenal domain) with THBS1 (By similarity). Interacts with THBS4 (via EGF-like 3; calcium-binding domain) which facilitates its processing, activation and nuclear translocation (PubMed:22682248). Interacts with XBP1 isoform 2; the interaction occurs in a ER stress-dependent manner (PubMed:17765680). {ECO:0000250, ECO:0000269|PubMed:17765680, ECO:0000269|PubMed:22682248}.
Tissue SpecificityUbiquitous.
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