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MB Sample ID: SA074710

Local Sample ID:R20_3
Subject ID:SU001140
Subject Type:Plant
Subject Species:Arabidopsis thaliana
Taxonomy ID:3702
Genotype Strain:Col-0/Cvi-0/Bur-0/MIB-22/Shahdara/Fei-0/Bor-4/Ts-1/Tsu-0/RRS-7/Est-1/Ler-1/C24/RRS-10/Bla-1/Br-0/Got-7/Kas-2/Lov-5/Mr-0/Pna-17/T540/Tamm-2/UKNW06-460/Ty-0

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Subject:

Subject ID:SU001140
Subject Type:Plant
Subject Species:Arabidopsis thaliana
Taxonomy ID:3702
Genotype Strain:Col-0/Cvi-0/Bur-0/MIB-22/Shahdara/Fei-0/Bor-4/Ts-1/Tsu-0/RRS-7/Est-1/Ler-1/C24/RRS-10/Bla-1/Br-0/Got-7/Kas-2/Lov-5/Mr-0/Pna-17/T540/Tamm-2/UKNW06-460/Ty-0

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
R20_3SA074710FL011544Pna-17ecotype
R20_3SA074710FL011544roottissue

Collection:

Collection ID:CO001134
Collection Summary:The ABRC stock IDs are stored in the uploaded data folder. Sterilized seeds were kept for 2 days at 4 degreeC in the dark, and then sown on Murashige and Skoog (MS) medium agar containing 1% sucrose. All plates were placed in a growth chamber at 22 degreeC under a 16-hr light (approximately 40 micro mol/(sec m^2)/8-hr dark cycle. After 3-week cultivation the plants were harvested and shoot and root parts were immediately frozen in liquid nitrogen and lyophilized at -55 degreeC. Lyophilized samples were stored at -80 degreeC until use. Three technical replicates for each strain were prepared from quenched samples.
Sample Type:Plant

Treatment:

Treatment ID:TR001154
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP001147
Sampleprep Summary:Samples (3 mg) of dried plant tissues were weighted in a 2.0-ml microtube (Sarstedt). Their metabolites were extracted with 150 µl of 80% MeOH containing 2.5 µM lidocaine and 10-camphour sulfonic acid per mg of dry weight using a mixer-mill with zirconia beads [7 min at 18 Hz and 4 degree C (MM300; Retsch)]. After 10-min centrifugation at 17,800 g the supernatant was filtered using an HLB µElution plate (Waters).

Combined analysis:

Analysis ID AN001783 AN001784
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity BEH C18 (100 x 2.1mm,1.7um) Waters Acquity BEH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 S QTOF Waters Synapt G2 S QTOF
Ion Mode POSITIVE NEGATIVE
Units Peak intensity Peak intensity

Chromatography:

Chromatography ID:CH001260
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:99.5% solvent A/0.5% solvent B at 0 min, 99.5%A/0.5%B at 0.1 min, 20%A/80%B at 10 min, 0.5%A/99.5%B at 10.1 min, 0.5%A/99.5%B at 12.0 min, 99.5%A/0.5%B at 12.1 min and 99.5%A/0.5%B at 15.0 min
Flow Rate:0.3 ml/min at 0 min, 0.3 ml/min at 10 min, 0.4 ml/min at 10.1 min, 0.4 ml/min at 14.4 min, and 0.3 ml/min at 14.5 min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS001647
Analysis ID:AN001783
Instrument Name:Waters Synapt G2 S QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Voltage:+3.00 kV
Collision Energy:6 V
Source Temperature:120
Desolvation Gas Flow:800 l/hr
Desolvation Temperature:450 degree C
Scan Range Moverz:50 - 1500
  
MS ID:MS001648
Analysis ID:AN001784
Instrument Name:Waters Synapt G2 S QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Voltage:-2.75 kV
Collision Energy:6 V
Source Temperature:120
Desolvation Gas Flow:800 l/hr
Desolvation Temperature:450 degree C
Scan Range Moverz:50 - 1500
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