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MB Sample ID: SA117894
| Local Sample ID: | S51 |
| Subject ID: | SU001494 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 59 ± 14.6 |
| Gender: | Male and female |
| Human Ethnicity: | Asian |
| Human Medications: | All with aspirin and clopidogrel |
| Human Smoking Status: | 22.2% were smokers |
| Human Inclusion Criteria: | The inclusion criteria were hospitalized CAD patients with stable angina. |
| Human Exclusion Criteria: | Patient with the lesion of > 90% stenosis or total occlusion, active inflammation (High sensitive C-reactive protein [hsCRP] >1.0 mg/dl), renal disease (serum creatinine > 3.0 mg/dl or ongoing hemodialysis), the onset of cancer in the previous five years, coronary artery bypass grafting, contraindications to iodine media, and severe hepatic insufficiency. |
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Subject:
| Subject ID: | SU001494 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 59 ± 14.6 |
| Gender: | Male and female |
| Human Ethnicity: | Asian |
| Human Medications: | All with aspirin and clopidogrel |
| Human Smoking Status: | 22.2% were smokers |
| Human Inclusion Criteria: | The inclusion criteria were hospitalized CAD patients with stable angina. |
| Human Exclusion Criteria: | Patient with the lesion of > 90% stenosis or total occlusion, active inflammation (High sensitive C-reactive protein [hsCRP] >1.0 mg/dl), renal disease (serum creatinine > 3.0 mg/dl or ongoing hemodialysis), the onset of cancer in the previous five years, coronary artery bypass grafting, contraindications to iodine media, and severe hepatic insufficiency. |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S51 | SA117894 | FL014837 | Angina | Disease |
Collection:
| Collection ID: | CO001489 |
| Collection Summary: | After an overnight fast, venous blood was collected from each patient at 48 h after PCI. Plasma was separated by centrifugation at 900 g for 10 minutes at room temperature within one hour of collection. The resulting fresh lithium-heparin plasma was transferred to new tubes and stored at -80 ˚C for further analysis. |
| Sample Type: | Blood (plasma) |
| Collection Frequency: | Once at 48 h after percutaneous coronary intervention (PCI) |
| Storage Conditions: | -80℃ |
| Collection Vials: | Lithium-heparin tubes |
| Collection Tube Temp: | Room temperature |
Treatment:
| Treatment ID: | TR001509 |
| Treatment Summary: | This is an observational cohort study. No treatment is involved. |
Sample Preparation:
| Sampleprep ID: | SP001502 |
| Sampleprep Summary: | Four volumes (400 µL) of prechilled (-20 ˚C) extraction buffer containing acetonitrile and methanol (50: 50, v/v) were added to 100 µL of plasma, vortexed vigorously, and incubated on crushed ice for 10 min. The mixture was then centrifuged at 16,000 g for 10 min at 4 ˚C. The supernatants containing the extracted metabolites were transferred to labeled cryotubes and stored at -80 ˚C for metabolomic analysis. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN002375 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Shimadzu 20AD |
| Column | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH001743 |
| Chromatography Summary: | Metabolomic analysis was performed on a Shimadzu LC-20A ultra-high-performance liquid chromatography coupled with SCIEX QTRAP 6500+ triple quadrupole mass spectrometer (LC-MS/MS) using a broad-spectrum targeted metabolomic approach. Ten µL of the extract was injected into a 250 mm × 2.0 mm, 4 µm polymer-based NH2 HPLC column (Asahipak NH2P-40 2E, Showa Denko America, Inc., NY) held at 25°C for chromatographic separation. The mobile phase was solvent A: 95% water, 5% acetonitrile with 20 mM NH4OAC, and 20 mM NH4OH (pH 9.4); solvent B: 100% acetonitrile. Separation was achieved using the following gradient: 0-3.5 min: 95% B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 14-30 min: 0% B, 31-41 min: 95% B, 41.1 min: end. The flow rate was 250 µL/min. |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
| Column Temperature: | 25 ℃ |
| Flow Gradient: | 0-3.5 min: 95% B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 14-30 min: 0% B, 31-41 min: 95% B, 41.1 min: end. |
| Flow Rate: | 250 µL/min |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium hydroxide, pH 9.4 (adjusted by formic acid) |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002217 |
| Analysis ID: | AN002375 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | A total of 606 metabolites covering the major metabolic pathways were targeted by scheduled multiple reaction monitoring (sMRM) under Analyst v1.6.2 software control in both negative and positive mode with rapid polarity switching (20 ms). Nitrogen was used for curtain gas (set to 30), collision gas (set to high), ion source gas 1 and 2 (set to 35). The source temperature was set to 500 ˚C. Spray voltage was set to -4500 V for negative mode and 5500 V for positive mode. |
| Ion Mode: | UNSPECIFIED |
