Summary of Study ST000098
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000090. The data can be accessed directly via it's Project DOI: 10.21228/M85K56 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000098 |
Study Title | Heatshock response of C. elegans using IROA (II) |
Institute | University of Florida |
Department | Department of Biochemistry and Molecular Biology and Southeastern Center for Integrated Metabolomics (SECIM) |
Laboratory | Arthur Edison |
Last Name | Edison |
First Name | Arthur |
Address | R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
aedison@ufl.edu | |
Phone | 352-392-4535 |
Submit Date | 2014-09-15 |
Num Groups | 1 |
Total Subjects | 1 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Uploaded File Size | 978 M |
Analysis Type Detail | NMR |
Release Date | 2015-11-04 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000090 |
Project DOI: | doi: 10.21228/M85K56 |
Project Title: | 13C Metabolomics: NMR and IROA for Unknown Identification |
Project Summary: | Demonstrate a method using high-sensitivity NMR to identify an unknown metabolite from a fraction isolated from an IROA LC-MS experiment. |
Institute: | University of Florida |
Department: | Department of Biochemistry and Molecular Biology and Southeastern Center for Integrated Metabolomics (SECIM) |
Laboratory: | Edison |
Last Name: | Edison |
First Name: | Arthur |
Email: | aedison@ufl.edu |
Phone: | 352-392-4535 |
Subject:
Subject ID: | SU000117 |
Subject Type: | Invertebrate |
Subject Species: | Caenorhabditis elegans |
Taxonomy ID: | 6239 |
Genotype Strain: | N2 |
Species Group: | Invertebrate |
Factors:
Subject type: Invertebrate; Subject species: Caenorhabditis elegans (Factor headings shown in green)
mb_sample_id | local_sample_id | Fractionation |
---|---|---|
SA005497 | HSP_F9_ms2_neg.raw | fraction 4-4.5 min |
SA005498 | HSP_F9_pos.raw | fraction 4-4.5 min |
SA005495 | HSP_F10_pos.raw | fraction 4.5-5 min |
SA005496 | HSP_F10_ms2_neg.raw | fraction 4.5-5 min |
SA005499 | HSP_ms2_pos.raw | Unfractionated |
SA005500 | HSP_pos.raw | Unfractionated |
Showing results 1 to 6 of 6 |
Collection:
Collection ID: | CO000100 |
Collection Summary: | Samples were already collected. |
Collection Protocol Filename: | NMR_Methods.docx LCMSMethod.docx |
Sample Type: | Worms |
Treatment:
Treatment ID: | TR000118 |
Treatment Summary: | No further treatment |
Sample Preparation:
Sampleprep ID: | SP000113 |
Sampleprep Summary: | Samples were mixed together and resuspended in 120 ?L of water. |
Sampleprep Protocol Filename: | NMR_Methods.docx LCMSMethod.docx |
Combined analysis:
Analysis ID |
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Analysis type |
Chromatography type |
Chromatography system |
Column |
MS Type |
MS instrument type |
MS instrument name |
Ion Mode |
Units |
MS:
MS ID: | MS000131 |
Analysis ID: | AN000157 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HSP_pos.raw, HSP_F9_pos.raw, HSP_F10_pos.raw |
Ion Mode: | POSITIVE |
Fragmentation Method: | Full MS |
Resolution Setting: | 70000 |
Scan Range Moverz: | 70-1000 |
MS ID: | MS000132 |
Analysis ID: | AN000158 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HSP_ms2_pos.raw |
Ion Mode: | POSITIVE |
Fragmentation Method: | Full MS/DD-MS2 (TOPN) |
Resolution Setting: | 70,000, 17,500 |
Scan Range Moverz: | 70-1000 |
MS ID: | MS000133 |
Analysis ID: | AN000159 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HSP_F10_ms2_neg.raw, HSP_F9_ms2_neg.raw |
Ion Mode: | NEGATIVE |
Fragmentation Method: | Full MS/DD-MS2 (TOPN) |
Resolution Setting: | 70,000, 17,500 |
Scan Range Moverz: | 70-1000 |