Summary of Study ST000229

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000185. The data can be accessed directly via it's Project DOI: 10.21228/M8BS32 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000229
Study TitleAnalysis of Dried Blood Spots by Paper Spray Ionization - MS
Study TypeAnalysis of Dried Blood Spots by Paper Spray Ionization - MS
Study SummaryConcentrations of cocaine (6, 5,3,2,1.5,1,0.75,0.5,0.25,0.125, 0.062, and 0.031 ppm) were spiked into whole human blood for PS-MS analysis. A 10 uL volume of blood/analyte mixture was allowed to dry on Whatman Grade 2 filter paper. A 1ppm concentration Cocaine-d3 was used as an internal standard. Parameters of the paper spray system were optimized, including spray voltage, capillary temperature and solvent. The internal standard was added on top of the dried blood spot sample. Wide isolation tandem MS was used to generate a calibration curve.
Institute
University of Florida
DepartmentChemistry
LaboratoryChemistry Lab Building
Last NameDhummakupt
First NameElizabeth
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emailemuffly@ufl.edu
Phone352-392-0515
Submit Date2015-07-16
Num Groups11
Total Subjects154
Study Comments11 concentrations were run as 7 samples/concentration for 2 days
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Uploaded File Size64 MB
Analysis Type DetailMS
Release Date2017-10-03
Release Version1
Elizabeth Dhummakupt Elizabeth Dhummakupt
https://dx.doi.org/10.21228/M8BS32
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000342
Analysis type MS
Chromatography type
Chromatography system
Column
MS Type Paper spray ionization
MS instrument type Ion trap
MS instrument name Thermo Deca LCQ
Ion Mode POSITIVE
Units Avg Intensity
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