Summary of Study ST000246
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000246 |
Study Title | Lipid Extraction Efficiency Comparison |
Study Type | Lipid Extraction Efficiency Comparison |
Study Summary | Aliquots of Jurkat T-lymphocyte cells were extracted using the Folch or Matyash method at different total volumes and run in triplicate to calculate the extraction efficiency for each method. |
Institute | University of Florida |
Department | Dept. of Chemistry/SECIM |
Laboratory | Biomedical Mass Spectrometry Lab |
Last Name | Ulmer |
First Name | Candice |
Address | R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
czulmer@chem.ufl.edu | |
Phone | (352) 392-0515 |
Submit Date | 2015-09-15 |
Num Groups | 4 |
Total Subjects | 12 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Uploaded File Size | 2.2 G |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000384 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS |
Column | Supelco Analytical Titan C18 |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH000276 |
Chromatography Summary: | Reversed Phase |
Methods Filename: | m_lipid_pos_switch_100 |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Supelco Analytical Titan C18 |
Column Pressure: | 1241 bar (max) |
Column Temperature: | 30 C |
Flow Gradient: | 32% B at 0 min, 40% B at 1 min, a hold at 40% B until 1.5 min, 45% B at 4 min, 50% B at 5 min, 60% B at 8 min, 70% B at 11 min, and 80% B at 14 min |
Flow Rate: | 0.5 mL/min |
Injection Temperature: | 4 C |
Sample Injection: | 2 μL |
Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
Analytical Time: | 21 min |
Randomization Order: | 3,10,12,11,8,6,5,4,7,9,2,1 |
Chromatography Type: | Reversed phase |