Summary of Study ST000269
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000213. The data can be accessed directly via it's Project DOI: 10.21228/M8VP42 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000269 |
| Study Title | Diacylglyceride and Ceramide analysis in TFD mice |
| Study Type | diet/age comparison |
| Study Summary | Mice were fed with a TFD for 8 or 24 weeks to induce NAFLD or NASH, respectively. Targeted analyses examined diacylglycerols and ceramides in 6-8 mice per group with 4 groups including 8 week control, 24 week control, 8 week TFD, and 24 week TFD. Samples were analyzed via UHPLC-HRMS |
| Institute | University of Florida |
| Department | Chemistry |
| Laboratory | Yost Laboratory |
| Last Name | Patterson |
| First Name | Rainey |
| Address | 214 Leigh Hall, University of Florida, Gainesville, FL 32611 |
| rpatterson@chem.ufl.edu | |
| Phone | 392-352-0551 |
| Submit Date | 2015-10-22 |
| Num Groups | 4 |
| Total Subjects | 28 |
| Raw Data Available | No |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-07-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000431 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | nmol/g liver protein |
Chromatography:
| Chromatography ID: | CH000301 |
| Chromatography Summary: | Waters Acquity BEH C18 (2.1 x 50 mm, 1.7 um) with guard was used to separate compounds with mobile phases made of isopropanol, acetonitrile, and water. |
| Methods Filename: | REP_18 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 30 C |
| Flow Gradient: | 32% B to 98% B over 18 mins |
| Flow Rate: | 0.5 mL/min |
| Injection Temperature: | 4 C |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Analytical Time: | 18 mins |
| Capillary Voltage: | 350 C |
| Time Program: | 0-1 min: 32%B --> 1-1.5 min: 40% B --> 4 min: 45% B --> 5 min: 50% B --> 8 min: 60% B --> 11 min: 70% B --> 14 min: 80% B --> 16-17 min: 100%B --> 18 min: 32%B --> 21 min: 32% B |
| Strong Wash Solvent Name: | 1:1:1 isopropanol:acetonitrile:water |
| Strong Wash Volume: | 100 uL |
| Chromatography Type: | Reversed phase |
