Summary of Study ST000362
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000288. The data can be accessed directly via it's Project DOI: 10.21228/M8TC7S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000362 |
| Study Title | Associations between 69 Sphingolipids and Emphysema, Chronic Bronchitis, Exacerbations, and FEV1/FVC |
| Study Type | Association Study |
| Study Summary | One hundred twenty-nine current and former smokers from the COPDGene cohort had 69 distinct sphingolipid species detected in plasma by targeted mass spectrometry. Of these, 23 were also measured in 131 plasma samples (117 independent subjects) using an untargeted platform in an independent laboratory. Regression analysis with adjustment for clinical covariates, correction for false discovery rate, and metaanalysis were used to test associations between COPD subphenotypes and sphingolipids. Peripheral blood mononuclear cells were used to test associations between sphingolipid gene expression and plasma sphingolipids. |
| Institute | National Jewish Health |
| Department | Medicine |
| Laboratory | Bowler |
| Last Name | Jacobson |
| First Name | Sean |
| Address | 1400 Jackson St, Denver, CO 80206 |
| jacobsons@njhealth.org | |
| Phone | (303) 398-1355 |
| Submit Date | 2016-03-10 |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-09-23 |
| Release Version | 1 |
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Combined analysis:
| Analysis ID | AN000594 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 10A |
| Column | Thermo Betasil C18 |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Shimadzu 10A |
| Ion Mode | POSITIVE |
| Units | Peak Area Ratio |
Chromatography:
| Chromatography ID: | CH000425 |
| Chromatography Summary: | A modified Bligh-Dyer extraction method was used in the presence of internal standards (IS). Sample analysis was performed in four batches with a Shimadzu 10A HPLC system coupled to a TSQ Quantum Ultra triple quadrupole mass spectrometer operated in SRM mode under ESI(+). A Thermo Betasil C18 column was employed to achieve the optimal sensitivity and separation. |
| Instrument Name: | Shimadzu 10A |
| Column Name: | Thermo Betasil C18 |
| Chromatography Type: | Reversed phase |
