Summary of Study ST000617
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000451. The data can be accessed directly via it's Project DOI: 10.21228/M8RP6W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000617 |
Study Title | Validation of the application of targeted metabolomic appraoch in the diagnosis of CFS |
Study Type | Plasma metabolomic profiling |
Study Summary | This study was to validate the utility of the developed targeted metabolomic method in the diagnosis of chronic fatigue syndrome (CFS). Clinical validation consisted of a cohort of 20 male CFS (53 ± 2.8 years old, mean ± SEM, range 21-67 y) and 18 male controls (53 ± 3.5 years old, mean ± SEM, range 23-69 y), who were enrolled in a previous study (Naviaux et al. 2016). These plasma samples were stored in -80 ºC for about 1.5 years and reanalyzed on a different LC-MS/MS system by a different investigator. |
Institute | University of California, San Diego |
Department | Department of Medicine |
Laboratory | The Mitochondrial and Metabolic Disease Center |
Last Name | Naviaux |
First Name | Robert |
Address | 214 Dikinson Street, CTF-C102, San Diego, CA, 92103 |
rnaviaux@ucsd.edu | |
Phone | 619-993-2904 |
Submit Date | 2017-05-17 |
Num Groups | 2 |
Total Subjects | 38 |
Num Males | 38 |
Study Comments | These plasma samples were stored in -80 ºC for about 1.5 years and reanalyzed on a different LC-MS/MS system by a different investigator. |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2017-06-17 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000946 | AN000947 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Shimadzu 20AD | Shimadzu 20AD |
Column | Shodex NH2P-40 2E (250× 2.0 mm) | Shodex NH2P-40 2E (250× 2.0 mm) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | NEGATIVE | POSITIVE |
Units | AUC | AUC |
Chromatography:
Chromatography ID: | CH000674 |
Chromatography Summary: | hydrophilic interaction chromatography (HILIC) on shodex column |
Instrument Name: | Shimadzu 20AD |
Column Name: | Shodex NH2P-40 2E (250× 2.0 mm) |
Column Pressure: | 900~2800 psi |
Column Temperature: | 25 C |
Flow Gradient: | 0-3.5 min: 95%B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 13.5–35 min: 0% B, 36–46 min: 95% B, 46.1 min: end. |
Flow Rate: | 0.2 ml/min |
Injection Temperature: | room temperature |
Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium carbonate; 38 mM ammonium hydroxide, pH 9.8 |
Solvent B: | 100% acetonitrile |
Analytical Time: | 46.1 min |
Oven Temperature: | 25 C |
Sample Loop Size: | 10 ul |
Sample Syringe Size: | 100 ul |
Chromatography Type: | HILIC |