Summary of Study ST001420
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000974. The data can be accessed directly via it's Project DOI: 10.21228/M8SQ55 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001420 |
| Study Title | Metabolomic analysis of patients with recurrent angina |
| Study Type | Case and control studies |
| Study Summary | This is a prospective study. After an overnight fast, venous blood was collected from patients with PCI. The patients were discharged after the blood draw. They were followed up every 30 days for angina recurrence up to 270 days (9 months). The samples were grouped into two groups: recurrent angina and angina-free based on the follow-up results at 9 months. |
| Institute | University of California, San Diego |
| Last Name | Cui |
| First Name | Song |
| Address | 9500 Gilman Drive, San Diego, CA, 92093, USA |
| kli@ucsd.edu | |
| Phone | 8582304220 |
| Submit Date | 2020-06-30 |
| Num Groups | 2 |
| Total Subjects | 750 |
| Num Males | 365 |
| Num Females | 385 |
| Release Date | 2020-07-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002375 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Shimadzu 20AD |
| Column | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH001743 |
| Chromatography Summary: | Metabolomic analysis was performed on a Shimadzu LC-20A ultra-high-performance liquid chromatography coupled with SCIEX QTRAP 6500+ triple quadrupole mass spectrometer (LC-MS/MS) using a broad-spectrum targeted metabolomic approach. Ten µL of the extract was injected into a 250 mm × 2.0 mm, 4 µm polymer-based NH2 HPLC column (Asahipak NH2P-40 2E, Showa Denko America, Inc., NY) held at 25°C for chromatographic separation. The mobile phase was solvent A: 95% water, 5% acetonitrile with 20 mM NH4OAC, and 20 mM NH4OH (pH 9.4); solvent B: 100% acetonitrile. Separation was achieved using the following gradient: 0-3.5 min: 95% B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 14-30 min: 0% B, 31-41 min: 95% B, 41.1 min: end. The flow rate was 250 µL/min. |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
| Column Temperature: | 25 ℃ |
| Flow Gradient: | 0-3.5 min: 95% B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 14-30 min: 0% B, 31-41 min: 95% B, 41.1 min: end. |
| Flow Rate: | 250 µL/min |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium hydroxide, pH 9.4 (adjusted by formic acid) |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
