Summary of Study ST002344
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001502. The data can be accessed directly via it's Project DOI: 10.21228/M8JD81 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002344 |
| Study Title | The impact of dietary serine/glycine restriction and myriocin treatment on hepatic sphingolipid diversity during prolonged high fat diet feeding. |
| Study Summary | We wanted to determine the impact of dietary serine/glycine restriction on hepatic sphingolipidome. |
| Institute | Salk Institute for Biological Studies |
| Department | Molecular and Cell Biology Laboratory |
| Laboratory | Metallo Lab |
| Last Name | Handzlik |
| First Name | Michal |
| Address | 10010 N Torrey Pines Rd, La Jolla, California, 92037, USA |
| mhandzlik@salk.edu | |
| Phone | 2069601945 |
| Submit Date | 2022-11-11 |
| Num Groups | LFD, -SG LFD, HFD, -SG HFD, LFD+Veh, HFD+Veh, -SG HFD+Veh, -SG HFD+Myr |
| Total Subjects | 80 |
| Num Males | 80 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003828 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | 6460C |
| Column | Peeke Scientific Spectra 3um C8SR (150 x 3mm) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6460 QQQ |
| Ion Mode | POSITIVE |
| Units | normalized ion count/mg protein |
Chromatography:
| Chromatography ID: | CH002833 |
| Chromatography Summary: | The gradient elution program consisted of the following profile: 0 min, 82% B; 3 min, 82% B; 4 min, 90% B, 18 min, 99% B; 25 min, 99%, 27 min, 82% B, 30 min, 82% B. |
| Instrument Name: | 6460C |
| Column Name: | Peeke Scientific Spectra 3um C8SR (150 x 3mm) |
| Column Temperature: | 40 |
| Flow Rate: | 0.5 ml/min |
| Solvent A: | 100% water; 0.2% formic acid; 2 mM ammonium formate |
| Solvent B: | 100% methanol; 0.2% formic acid; 1 mM ammonium formate |
| Chromatography Type: | Reversed phase |
