Summary of Study ST002367
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001521. The data can be accessed directly via it's Project DOI: 10.21228/M83707 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002367 |
| Study Title | Single cell lipidome analysis of phosphatidylcholines and spingomyelins from prostate cells |
| Study Type | Quantitative single cell lipidomics |
| Study Summary | We have developed a high-throughput method for the detection and quantification of a wide range of phosphatidylcholine (PC) and sphingomyelin (SM) species from single cells that combines fluorescence-assisted cell sorting (FACS) with automated chip-based nanoelectrospray ionization (nanoESI) and shotgun lipidomics. Using this method we can detect and perform relative quantitation on more than >50 different PC and SM species from immortalised human cells, and can easily distinguish between tumorigenic and non-tumorigenic prostate cell lines. |
| Institute | Victor Chang Cardiac Research Institute |
| Laboratory | Cellular Bioenergetics Laboratory |
| Last Name | Hancock |
| First Name | Sarah |
| Address | Lowy Packer Building, 405 Liverpool Street, Darlinghurst, NSW, 2010, Australia |
| s.hancock@victorchang.edu.au | |
| Phone | +61414537526 |
| Submit Date | 2022-10-30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2023-10-19 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002449 |
| Collection Summary: | Samples were obtained from established immortalised adherent human tumorigenic (DU145, LNCaP & PC3) and non-tumorigenic (PNT1) cell lines. Cells were cultured in RPMI (R5886, Sigma-Aldrich) supplemented with 2 mM L-glutamine and 10% heat-inactivated fetal calf serum and incubated at 37°C in 5% CO2. Cell media was changed every three days and cells were passaged regularly at ~80-90% confluency by trypsinization. Cell lines were regularly screened for mycoplasma infection. |
| Sample Type: | Prostate |
