Summary of Study ST002178
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001386. The data can be accessed directly via it's Project DOI: 10.21228/M8K11W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002178 |
| Study Title | Age-independent Cardiac Protection by Pharmacological Activation of Beclin-1 During Endotoxemia and Its Association with Energy Metabolic Reprograming in Myocardium — A Targeted Metabolomics Study |
| Study Summary | Background: We previously showed that Beclin-1-dependent autophagy is cardiac protective in a rodent model of endotoxemia using young adult mice. In this report, we compared the potential therapeutic effects of pharmacological Beclin-1 activating peptide, TB-peptide, on the cardiac outcomes of young adult and aged mice during endotoxemia. We further examined alterations in myocardial metabolism induced by lipopolysaccharide (LPS) challenge with and without the TB-peptide treatment. Methods and Results: C57BL/6J mice of 10-week and 24-month-old were challenged by LPS at doses at which cardiac dysfunction occurred. Following the treatment of TB-peptide or control vehicle, heart contractility, circulating cytokines, and myocardial autophagy were evaluated. A targeted metabolomics assay was applied to analyze cardiac metabolism. TB-peptide boosted autophagic response, attenuated cytokine production, and improved cardiac performance in both young and aged mice during endotoxemia. A targeted metabolomics assay was designed to detect a pool of 361 known metabolites, of which 156 were detected in at least one of the heart tissue samples. LPS-induced impairments were found in glucose and amino acid (AA) metabolisms in mice of all ages, and TB-peptide provided ameliorative effects to rescue these alterations. However, lipid metabolites were upregulated in the young group but moderately downregulated in the aged by LPS, suggesting an age-dependent response. TB-peptide mitigated LPS-mediated trend of lipids in the young mice but provided little effect on the aged ones. Conclusion: Pharmacological activation of Beclin-1 by TB-peptide protects the heart in both young and aged population during endotoxemia, suggest a therapeutic potential for sepsis-induced cardiomyopathy. Metabolomics analysis suggests that this age-independent protection by TB-peptide is associated with reprograming of energy production via glucose and AA metabolisms. |
| Institute | Loyola University Chicago Stritch School of Medicine |
| Department | Surgery |
| Last Name | Zang |
| First Name | Qun |
| Address | 2160 S. 1st Ave, Maywood, IL 60153 |
| qzang@luc.edu | |
| Phone | 708-327-2472 |
| Submit Date | 2022-05-23 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-06-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Age Group | Treatment |
|---|---|---|---|
| SA209208 | 2020-08-17_Zang-43_Box2Redo-Blank_NEG | blank | - |
| SA209209 | 2020-08-17_Zang-43_Box2Redo-Blank_NEG (3) | blank | - |
| SA209210 | 2020-08-17_Zang-43_Box2Redo-Blank_NEG (2) | blank | - |
| SA209211 | 2020-08-17_Zang-43_Box2Redo-Blank_NEG (4) | blank | - |
| SA209212 | 2020-08-17_Zang-43_Box2Redo-Blank_NEG (5) | blank | - |
| SA209213 | 2020-08-17_Zang-43_Box2Redo-Blank_NEG (6) | blank | - |
| SA209214 | 2020-08-17_Zang-43_Box2Redo-Blank0_NEG | blank | - |
| SA209215 | 2020-08-17_Zang-43_Box2Redo-Blank_POS | blank | - |
| SA209216 | 2020-08-17_Zang-43_Box2Redo-Blank_POS (6) | blank | - |
| SA209217 | 2020-08-17_Zang-43_Box2Redo-Blank_POS (5) | blank | - |
| SA209218 | 2020-08-17_Zang-43_Box2Redo-Blank_POS (4) | blank | - |
| SA209219 | 2020-08-17_Zang-43_Box2Redo-Blank_POS (2) | blank | - |
| SA209220 | 2020-08-17_Zang-43_Box2Redo-Blank_POS (3) | blank | - |
| SA209221 | 2020-08-17_Zang-43_Box2Redo-Dummy_NEG | instrument quality control | - |
| SA209222 | 2020-08-17_Zang-43_Box2Redo-QC(I)#5_NEG | instrument quality control | - |
| SA209223 | 2020-08-17_Zang-43_Box2Redo-QC(I)#1_NEG | instrument quality control | - |
| SA209224 | 2020-08-17_Zang-43_Box2Redo-QC(I)#1_POS | instrument quality control | - |
| SA209225 | 2020-08-17_Zang-43_Box2Redo-Dummy_POS | instrument quality control | - |
| SA209226 | 2020-08-17_Zang-43_Box2Redo-QC(I)#3_POS | instrument quality control | - |
| SA209227 | 2020-08-17_Zang-43_Box2Redo-QC(I)#5_POS | instrument quality control | - |
| SA209228 | 2020-08-17_Zang-43_Box2Redo-QC(I)#4_POS | instrument quality control | - |
| SA209229 | 2020-08-17_Zang-43_Box2Redo-QC(I)#2_NEG | instrument quality control | - |
| SA209230 | 2020-08-17_Zang-43_Box2Redo-QC(I)#4_NEG | instrument quality control | - |
| SA209231 | 2020-08-17_Zang-43_Box2Redo-QC(I)#3_NEG | instrument quality control | - |
| SA209232 | 2020-08-17_Zang-43_Box2Redo-QC(I)#2_POS | instrument quality control | - |
| SA209122 | 2020-08-17_Zang-43_Box2Redo-28_POS | Old | Ctrl |
| SA209123 | 2020-08-17_Zang-43_Box2Redo-21_POS | Old | Ctrl |
| SA209124 | 2020-08-17_Zang-43_Box2Redo-34_POS | Old | Ctrl |
| SA209125 | 2020-08-17_Zang-43_Box2Redo-23_NEG | Old | Ctrl |
| SA209126 | 2020-08-17_Zang-43_Box2Redo-28_NEG | Old | Ctrl |
| SA209127 | 2020-08-17_Zang-43_Box2Redo-34_NEG | Old | Ctrl |
| SA209128 | 2020-08-17_Zang-43_Box2Redo-38_POS | Old | Ctrl |
| SA209129 | 2020-08-17_Zang-43_Box2Redo-23_POS | Old | Ctrl |
| SA209130 | 2020-08-17_Zang-43_Box2Redo-38_NEG | Old | Ctrl |
| SA209131 | 2020-08-17_Zang-43_Box2Redo-21_NEG | Old | Ctrl |
| SA209132 | 2020-08-17_Zang-43_Box2Redo-26_NEG | Old | LPS1 |
| SA209133 | 2020-08-17_Zang-43_Box2Redo-1_NEG | Old | LPS1 |
| SA209134 | 2020-08-17_Zang-43_Box2Redo-37_NEG | Old | LPS1 |
| SA209135 | 2020-08-17_Zang-43_Box2Redo-26_POS | Old | LPS1 |
| SA209136 | 2020-08-17_Zang-43_Box2Redo-40_NEG | Old | LPS1 |
| SA209137 | 2020-08-17_Zang-43_Box2Redo-7_NEG | Old | LPS1 |
| SA209138 | 2020-08-17_Zang-43_Box2Redo-37_POS | Old | LPS1 |
| SA209139 | 2020-08-17_Zang-43_Box2Redo-1_POS | Old | LPS1 |
| SA209140 | 2020-08-17_Zang-43_Box2Redo-40_POS | Old | LPS1 |
| SA209141 | 2020-08-17_Zang-43_Box2Redo-7_POS | Old | LPS1 |
| SA209150 | 2020-08-17_Zang-43_Box2Redo-16_NEG | Old | LPS1_peptide |
| SA209151 | 2020-08-17_Zang-43_Box2Redo-16_POS | Old | LPS1_peptide |
| SA209142 | 2020-08-17_Zang-43_Box2Redo-27_NEG | Old | LPS1-peptide |
| SA209143 | 2020-08-17_Zang-43_Box2Redo-27_POS | Old | LPS1-peptide |
| SA209144 | 2020-08-17_Zang-43_Box2Redo-32_POS | Old | LPS1-peptide |
| SA209145 | 2020-08-17_Zang-43_Box2Redo-39_POS | Old | LPS1-peptide |
| SA209146 | 2020-08-17_Zang-43_Box2Redo-39_NEG | Old | LPS1-peptide |
| SA209147 | 2020-08-17_Zang-43_Box2Redo-10_NEG | Old | LPS1-peptide |
| SA209148 | 2020-08-17_Zang-43_Box2Redo-32_NEG | Old | LPS1-peptide |
| SA209149 | 2020-08-17_Zang-43_Box2Redo-10_POS | Old | LPS1-peptide |
| SA209152 | 2020-08-17_Zang-43_Box2Redo-43_NEG | Old | peptide |
| SA209153 | 2020-08-17_Zang-43_Box2Redo-8_NEG | Old | peptide |
| SA209154 | 2020-08-17_Zang-43_Box2Redo-3_NEG | Old | peptide |
| SA209155 | 2020-08-17_Zang-43_Box2Redo-3_POS | Old | peptide |
| SA209156 | 2020-08-17_Zang-43_Box2Redo-29_POS | Old | peptide |
| SA209157 | 2020-08-17_Zang-43_Box2Redo-43_POS | Old | peptide |
| SA209158 | 2020-08-17_Zang-43_Box2Redo-8_POS | Old | peptide |
| SA209159 | 2020-08-17_Zang-43_Box2Redo-29_NEG | Old | peptide |
| SA209233 | 2020-08-17_Zang-43_Box2Redo-QC(S)#3_POS | sample quality control | - |
| SA209234 | 2020-08-17_Zang-43_Box2Redo-QC(S)#1_POS | sample quality control | - |
| SA209235 | 2020-08-17_Zang-43_Box2Redo-QC(S)#2_POS | sample quality control | - |
| SA209236 | 2020-08-17_Zang-43_Box2Redo-QC(S)#5_NEG | sample quality control | - |
| SA209237 | 2020-08-17_Zang-43_Box2Redo-QC(S)#2_NEG | sample quality control | - |
| SA209238 | 2020-08-17_Zang-43_Box2Redo-QC(S)#3_NEG | sample quality control | - |
| SA209239 | 2020-08-17_Zang-43_Box2Redo-QC(S)#1_NEG | sample quality control | - |
| SA209240 | 2020-08-17_Zang-43_Box2Redo-QC(S)#4_NEG | sample quality control | - |
| SA209241 | 2020-08-17_Zang-43_Box2Redo-QC(S)#5_POS | sample quality control | - |
| SA209242 | 2020-08-17_Zang-43_Box2Redo-QC(S)#4_POS | sample quality control | - |
| SA209121 | 2020-08-17_Zang-43_Box2Redo-Blank0_POS | - | - |
| SA209160 | 2020-08-17_Zang-43_Box2Redo-17_NEG | Young | Ctrl |
| SA209161 | 2020-08-17_Zang-43_Box2Redo-30_NEG | Young | Ctrl |
| SA209162 | 2020-08-17_Zang-43_Box2Redo-19_NEG | Young | Ctrl |
| SA209163 | 2020-08-17_Zang-43_Box2Redo-5_POS | Young | Ctrl |
| SA209164 | 2020-08-17_Zang-43_Box2Redo-9_POS | Young | Ctrl |
| SA209165 | 2020-08-17_Zang-43_Box2Redo-19_POS | Young | Ctrl |
| SA209166 | 2020-08-17_Zang-43_Box2Redo-9_NEG | Young | Ctrl |
| SA209167 | 2020-08-17_Zang-43_Box2Redo-31_NEG | Young | Ctrl |
| SA209168 | 2020-08-17_Zang-43_Box2Redo-5_NEG | Young | Ctrl |
| SA209169 | 2020-08-17_Zang-43_Box2Redo-30_POS | Young | Ctrl |
| SA209170 | 2020-08-17_Zang-43_Box2Redo-31_POS | Young | Ctrl |
| SA209171 | 2020-08-17_Zang-43_Box2Redo-17_POS | Young | Ctrl |
| SA209172 | 2020-08-17_Zang-43_Box2Redo-14_NEG | Young | LPS3 |
| SA209173 | 2020-08-17_Zang-43_Box2Redo-6_NEG | Young | LPS3 |
| SA209174 | 2020-08-17_Zang-43_Box2Redo-4_NEG | Young | LPS3 |
| SA209175 | 2020-08-17_Zang-43_Box2Redo-11_NEG | Young | LPS3 |
| SA209176 | 2020-08-17_Zang-43_Box2Redo-25_NEG | Young | LPS3 |
| SA209177 | 2020-08-17_Zang-43_Box2Redo-36_POS | Young | LPS3 |
| SA209178 | 2020-08-17_Zang-43_Box2Redo-36_NEG | Young | LPS3 |
| SA209179 | 2020-08-17_Zang-43_Box2Redo-11_POS | Young | LPS3 |
| SA209180 | 2020-08-17_Zang-43_Box2Redo-14_POS | Young | LPS3 |
| SA209181 | 2020-08-17_Zang-43_Box2Redo-6_POS | Young | LPS3 |
| SA209182 | 2020-08-17_Zang-43_Box2Redo-25_POS | Young | LPS3 |
| SA209183 | 2020-08-17_Zang-43_Box2Redo-4_POS | Young | LPS3 |
| SA209184 | 2020-08-17_Zang-43_Box2Redo-35_NEG | Young | LPS3_peptide |
| SA209185 | 2020-08-17_Zang-43_Box2Redo-20_POS | Young | LPS3_peptide |
