Summary of Study ST002503
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001617. The data can be accessed directly via it's Project DOI: 10.21228/M8PH89 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002503 |
| Study Title | Endothelial Cell CD36 Regulates Membrane Ceramide Formation, Exosome Fatty Acid Delivery to Tissues and Circulating Fatty Acid Levels |
| Study Type | Membrane ceramide and Fatty Acid Uptake |
| Study Summary | Endothelial cell (EC) CD36 controls tissue fatty acid (FA) uptake. Here we visualized FA transfer by ECs using click chemistry. Apical FA interaction with EC CD36 induces actin reorganization, caveolin-1 tyrosine 14 (Cav-1Y14) phosphorylation, and ceramide generation in caveolae, culminating in caveolae internalization into FA/CD36/ceramide vesicles that are secreted basolaterally as small extracellular vesicles, sEVs (exosomes). In transwells, ECs expressing fluorescent CD63 (exosomal marker) transfer FAs to underlying myotubes in CD63-labeled sEVs. In vivo FA-CD63-CD36 transfer from ECs to muscle is shown using a mouse with EC-specific expression of emGFP-CD63. CD36 depletion, actin-binding latrunculin-B, Src inhibition, Cav-1Y14 mutation, and membrane neutral sphingomyelinase inhibition by GW4869 helped map the FA-sEV pathway. Injecting GW4869 to mice reduced muscle FA uptake, raised plasma FAs and lowered glucose, mimicking prominent Cd36-/- phenotypes. The FA-sEV pathway we describe contributes to crosstalk between ECs and underlying cells and links regulation of membrane ceramide to blood FAs. |
| Institute | Washington University in St. Louis |
| Department | IM-Nutitrional Science |
| Laboratory | Abumrad Lab |
| Last Name | Palacios |
| First Name | Hector |
| Address | West Building 00201, St. Louis, Missouri, 63110, USA |
| hectorp@wustl.edu | |
| Phone | 314-362-5397 |
| Submit Date | 2023-03-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-03-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype | Treatment |
|---|---|---|---|
| SA251330 | KO3n_14_FA | Knock-out | Neg |
| SA251331 | KO1n_13_FA | Knock-out | Neg |
| SA251332 | KO3n_06_FA | Knock-out | Neg |
| SA251333 | KO1n_21_FA | Knock-out | Neg |
| SA251334 | KO3n_22_FA | Knock-out | Neg |
| SA251335 | KO1n_05 | Knock-out | Neg |
| SA251336 | KO3n_22 | Knock-out | Neg |
| SA251337 | KO1n_21 | Knock-out | Neg |
| SA251338 | KO1n_13 | Knock-out | Neg |
| SA251339 | KO3n_06 | Knock-out | Neg |
| SA251340 | KO3n_14 | Knock-out | Neg |
| SA251341 | KO1n_05_FA | Knock-out | Neg |
| SA251342 | KO1p_07_FA | Knock-out | Pos |
| SA251343 | KO3p_08_FA | Knock-out | Pos |
| SA251344 | KO3p_16_FA | Knock-out | Pos |
| SA251345 | KO1p_15_FA | Knock-out | Pos |
| SA251346 | KO1p_23_FA | Knock-out | Pos |
| SA251347 | KO1p_15 | Knock-out | Pos |
| SA251348 | KO3p_08 | Knock-out | Pos |
| SA251349 | KO3p_16 | Knock-out | Pos |
| SA251350 | KO3p_24 | Knock-out | Pos |
| SA251351 | KO1p_07 | Knock-out | Pos |
| SA251352 | KO3p_24_FA | Knock-out | Pos |
| SA251353 | KO1p_23 | Knock-out | Pos |
| SA251354 | WT3n_10_FA | Wild-type | Neg |
| SA251355 | WT3n_18_FA | Wild-type | Neg |
| SA251356 | WT1n_17_FA | Wild-type | Neg |
| SA251357 | WT1n_09_FA | Wild-type | Neg |
| SA251358 | WT1n_01_FA | Wild-type | Neg |
| SA251359 | WT3n_02 | Wild-type | Neg |
| SA251360 | WT1n_17 | Wild-type | Neg |
| SA251361 | WT1n_09 | Wild-type | Neg |
| SA251362 | WT1n_01 | Wild-type | Neg |
| SA251363 | WT3n_18 | Wild-type | Neg |
| SA251364 | WT3n_10 | Wild-type | Neg |
| SA251365 | WT3n_02_FA | Wild-type | Neg |
| SA251366 | WT1p_11 | Wild-type | Pos |
| SA251367 | WT1p_03 | Wild-type | Pos |
| SA251368 | WT3p_20 | Wild-type | Pos |
| SA251369 | WT3p_20_FA | Wild-type | Pos |
| SA251370 | WT1p_03_FA | Wild-type | Pos |
| SA251371 | WT1p_19_FA | Wild-type | Pos |
| SA251372 | WT3p_12_FA | Wild-type | Pos |
| SA251373 | WT3p_04 | Wild-type | Pos |
| SA251374 | WT3p_04_FA | Wild-type | Pos |
| SA251375 | WT1p_11_FA | Wild-type | Pos |
| SA251376 | WT1p_19 | Wild-type | Pos |
| SA251377 | WT3p_12 | Wild-type | Pos |
| Showing results 1 to 48 of 48 |
