Summary of Study ST003012
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001875. The data can be accessed directly via it's Project DOI: 10.21228/M8BX4T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003012 |
| Study Title | A High-Fat Eucaloric Diet Induces Reprometabolic Syndrome of Obesity in Normal Weight Women - metabolomics |
| Study Summary | Objective: We examined the effects of one month of a eucaloric, high-fat (48% of calories) diet (HFD) on gonadotropin secretion in normal weight women to interrogate the role of free fatty acids and insulin in mediating the relative hypogonadotropic hypogonadism of obesity. Methods: Eighteen eumenorrheic women (BMI 18-25 kg/m2) were studied in the early follicular phase of the menstrual cycle before and after exposure to a HFD with frequent blood sampling for LH and FSH, followed by an assessment of pituitary sensitivity to GnRH. Mass spectrometrybased plasma metabolomic analysis was also performed. Paired testing and time series analysis were performed as appropriate. Results: Mean endogenous LH (unstimulated) was significantly decreased after the HFD (4.3 ±1.0 vs 3.8 ± 1.0, P<0.01); mean unstimulated FSH was not changed. Both LH (10.1 ± 1.0 vs 7.2 ± 1.0, P<0.01), and FSH (9.5 ± 1.0 vs 8.8 ± 1.0, P<0.01) response to 75 ng/kg of GnRH were reduced after the HFD. Mean LH pulse amplitude and LH interpulse interval were unaffected by the dietary exposure. Eucaloric HFD exposure did not cause weight change. Plasma metabolomics confirmed adherence with elevation of fasting free fatty acids (especially long-chain mono-, poly- and highly-unsaturated fatty acids) by the last day of the HFD. Conclusion: One-month exposure to a HFD successfully induced key reproductive and metabolic features of Reprometabolic Syndrome in normal weight women. Dietary factors may underly the gonadotrope compromise seen in obesity related subfertility and that therapeutic dietary interventions, independent of weight loss, may be possible. |
| Institute | University of Colorado Denver |
| Last Name | Haines |
| First Name | Julie |
| Address | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
| julie.haines@cuanschutz.edu | |
| Phone | 3037243339 |
| Submit Date | 2023-12-14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | factor |
|---|---|---|
| SA327071 | DS1-27-49 | On-diet |
| SA327072 | DS1-27-02 | On-diet |
| SA327073 | DS1-27-43 | On-diet |
| SA327074 | DS1-27-41 | On-diet |
| SA327075 | DS1-27-52 | On-diet |
| SA327076 | DS1-27-56 | On-diet |
| SA327077 | DS1-27-54 | On-diet |
| SA327078 | DS1-27-38 | On-diet |
| SA327079 | DS1-27-46 | On-diet |
| SA327080 | DS1-27-11 | On-diet |
| SA327081 | DS1-27-35 | On-diet |
| SA327082 | DS1-27-05 | On-diet |
| SA327083 | DS1-27-14 | On-diet |
| SA327084 | DS1-27-08 | On-diet |
| SA327085 | DS1-27-17 | On-diet |
| SA327086 | DS1-27-26 | On-diet |
| SA327087 | DS1-27-29 | On-diet |
| SA327088 | DS1-27-20 | On-diet |
| SA327089 | DS1-27-36 | Post-diet |
| SA327090 | DS1-27-30 | Post-diet |
| SA327091 | DS1-27-39 | Post-diet |
| SA327092 | DS1-27-50 | Post-diet |
| SA327093 | DS1-27-47 | Post-diet |
| SA327094 | DS1-27-44 | Post-diet |
| SA327095 | DS1-27-27 | Post-diet |
| SA327096 | DS1-27-03 | Post-diet |
| SA327097 | DS1-27-21 | Post-diet |
| SA327098 | DS1-27-06 | Post-diet |
| SA327099 | DS1-27-09 | Post-diet |
| SA327100 | DS1-27-18 | Post-diet |
| SA327101 | DS1-27-15 | Post-diet |
| SA327102 | DS1-27-12 | Post-diet |
| SA327103 | DS1-27-45 | Pre-diet |
| SA327104 | DS1-27-42 | Pre-diet |
| SA327105 | DS1-27-48 | Pre-diet |
| SA327106 | DS1-27-55 | Pre-diet |
| SA327107 | DS1-27-37 | Pre-diet |
| SA327108 | DS1-27-53 | Pre-diet |
| SA327109 | DS1-27-51 | Pre-diet |
| SA327110 | DS1-27-40 | Pre-diet |
| SA327111 | DS1-27-19 | Pre-diet |
| SA327112 | DS1-27-07 | Pre-diet |
| SA327113 | DS1-27-04 | Pre-diet |
| SA327114 | DS1-27-01 | Pre-diet |
| SA327115 | DS1-27-10 | Pre-diet |
| SA327116 | DS1-27-13 | Pre-diet |
| SA327117 | DS1-27-28 | Pre-diet |
| SA327118 | DS1-27-25 | Pre-diet |
| SA327119 | DS1-27-16 | Pre-diet |
| SA327120 | DS1-27-34 | Pre-diet |
| Showing results 1 to 50 of 50 |
