Summary of Study ST003035

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001888. The data can be accessed directly via it's Project DOI: 10.21228/M8P728 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003035
Study TitleCentral Transcriptional Regulator Controls Growth and Carbon Storage under High Light Stress in Photosynthetic Microalgae Model Strains
Study TypeAlgae
Study SummaryCarbon capture efficiency and biochemical storage are some of the primary drivers of photosynthetic productivity and by extension crop yield. To elucidate the mechanisms governing yield phenotypes and carbon allocation regulatory elements, we selected two microalgae strains as simplified models of photosynthetic crops. The Picochlorum celeri TG2 isolate is one of the fastest growing algae and in this work is juxtaposed to a closely related, slower growing, isolate, TG1, of the same species with less than 2% genomic divergence. Through the application of a comprehensive systems biology light-stress response study, we observed a stark difference in carbon assimilation and storage rates, with the slower growing isolate accumulating almost three times the amount of starch compared to the fast-growing isolate. We characterized the carbon storage rates and allocation dynamics, with metabolic bottlenecks, and transport rates of intermediates underlying the variations in growth and composition in high light using instationary 13C-fluxomics experiments. High light stress analysis of transcriptomic dynamics during acclimation of the strains from low to high light identified a widespread response with up to 73% the annotated gene set significantly differentially expressed after only 1 hour. Broad transcriptional regulatory control was inferred by a rapid depletion of a global diel-responsive transcription factor closely related to a circadian-regulator in plants, as the single most distinct transcription factor. Transferring this factor to the slower variant increased yield, specific growth rate, and carbohydrate accumulation of the selected engineered strain, providing further evidence for a coordinating regulatory mechanism for this complex phenotype.
Institute
National Renewable Energy Lab
DepartmentBiosciences
LaboratoryLaurens Lab
Last NameLaurens
First NameLieve
Address15013 Denver West Paekway, Golden, CO 80401
Emaillieve.laurens@nrel.gov
Phone+1 720-273-6534
Submit Date2023-12-18
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-04-26
Release Version1
Lieve Laurens Lieve Laurens
https://dx.doi.org/10.21228/M8P728
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Picochlorum celeri (Factor headings shown in green)

mb_sample_id local_sample_id Strain Time (s)
SA328731TG1-MYB99_R3_0TG1-MYB99 0 (no label)
SA328732TG1-MYB99_R2_0TG1-MYB99 0 (no label)
SA328733TG1-MYB99_R1_0TG1-MYB99 0 (no label)
SA328734TG1-MYB99_R1_180TG1-MYB99 180
SA328735TG1-MYB99_R3_180TG1-MYB99 180
SA328736TG1-MYB99_R2_180TG1-MYB99 180
SA328737TG1-MYB99_R2_30TG1-MYB99 30
SA328738TG1-MYB99_R3_30TG1-MYB99 30
SA328739TG1-MYB99_R1_30TG1-MYB99 30
SA328740TG1-MYB99_R3_300TG1-MYB99 300
SA328741TG1-MYB99_R1_300TG1-MYB99 300
SA328742TG1-MYB99_R2_300TG1-MYB99 300
SA328743TG1-MYB99_R1_60TG1-MYB99 60
SA328744TG1-MYB99_R2_60TG1-MYB99 60
SA328745TG1-MYB99_R3_60TG1-MYB99 60
SA328746TG1-MYB99_R1_600TG1-MYB99 600
SA328747TG1-MYB99_R2_600TG1-MYB99 600
SA328748TG1-MYB99_R3_600TG1-MYB99 600
SA328749TG1_R3_0TG1 0 (no label)
SA328750TG1_R1_0TG1 0 (no label)
SA328751TG1_R2_0TG1 0 (no label)
SA328752TG1_R3_180TG1 180
SA328753TG1_R2_180TG1 180
SA328754TG1_R1_180TG1 180
SA328755TG1_R3_30TG1 30
SA328756TG1_R1_30TG1 30
SA328757TG1_R2_30TG1 30
SA328758TG1_R3_300TG1 300
SA328759TG1_R1_300TG1 300
SA328760TG1_R2_300TG1 300
SA328761TG1_R1_60TG1 60
SA328762TG1_R3_60TG1 60
SA328763TG1_R2_60TG1 60
SA328764TG1_R1_600TG1 600
SA328765TG1_R3_600TG1 600
SA328766TG1_R2_600TG1 600
SA328767TG2_R1_0TG2 0 (no label)
SA328768TG2_R3_0TG2 0 (no label)
SA328769TG2_R2_0TG2 0 (no label)
SA328770TG2_R2_180TG2 180
SA328771TG2_R1_180TG2 180
SA328772TG2_R3_180TG2 180
SA328773TG2_R3_30TG2 30
SA328774TG2_R2_30TG2 30
SA328775TG2_R1_30TG2 30
SA328776TG2_R1_300TG2 300
SA328777TG2_R3_300TG2 300
SA328778TG2_R2_300TG2 300
SA328779TG2_R2_60TG2 60
SA328780TG2_R1_60TG2 60
SA328781TG2_R3_60TG2 60
SA328782TG2_R3_600TG2 600
SA328783TG2_R2_600TG2 600
SA328784TG2_R1_600TG2 600
Showing results 1 to 54 of 54
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