Summary of Study ST001062
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000712. The data can be accessed directly via it's Project DOI: 10.21228/M8N965 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001062 |
| Study Title | Arabidopsis Nit1 knockout metabolomics |
| Study Summary | Glutathione (GSH) is a tripeptide that is implicated in various crucial physiological processes including redox buffering and protection against heavy metal toxicity. GSH is abundant in plants, with reported intracellular concentrations typically in the 1-10 millimolar range. Various aminotransferases can inadvertently transaminate the amino group of the γ-glutamyl moiety of GSH to produce deaminated glutathione (dGSH), a metabolite damage product. It was recently reported that an amidase known as Nit1 participates in dGSH breakdown in mammals and yeast. Plants have a hitherto uncharacterized homolog of the Nit1 amidase. We show that recombinant Arabidopsis Nit1 (At4g08790) has efficient amidase activity towards dGSH. Ablating the Arabidopsis Nit1 gene causes a massive accumulation of dGSH and other marked changes to the metabolome. All plant Nit1 sequences examined had predicted plastidial targeting peptides with a potential second start codon whose use would eliminate the targeting peptide. In vitro transcription/translation assays show that both potential translation start codons were used and subcellular localization of GFP fusions confirmed both cytosolic and plastidial localization. Further, we show that Arabidopsis enzymes convert GSH to dGSH at a rate of 2.8 pmol min-1 mg-1 in vitro. Our data demonstrate that plants have a dGSH repair system that is directed to at least two subcellular compartments via the use of alternative translation start sites. |
| Institute | University of California, Davis |
| Last Name | Folz |
| First Name | Jacob |
| Address | 451 Health Sciences Dr., Davis, CA, 95616 |
| jfolz@ucdavis.edu | |
| Phone | 7155636311 |
| Submit Date | 2018-09-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-03-06 |
| Release Version | 1 |
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Project:
| Project ID: | PR000712 |
| Project DOI: | doi: 10.21228/M8N965 |
| Project Title: | Nit1 Arabidopsis knockout |
| Project Type: | Untargeted Mass Spectrometry |
| Project Summary: | Arabidopsis tissue with knockout of Nit1 gene analyzed for disruptions in metabolism. Polar and non-polar metabolites were measured using HILIC chromatography and C18 chromatography |
| Institute: | University of California, Davis |
| Laboratory: | Oliver Fiehn |
| Last Name: | Folz |
| First Name: | Jacob |
| Address: | 451 Health Science Drive, Davis, CA 95616 |
| Email: | jfolz@ucdavis.edu |
| Phone: | 7155636311 |
| Funding Source: | MCB-1153491 |
| Contributors: | Thomas D. Niehaus, Jenelle Patterson, Danny C. Alexander, Jacob S. Folz, Michal Pyc, Brian S. MacTavish, Robert T. Mullen, Oliver Fiehn, Steven D. Bruner, and Andrew D. Hanson |
