Summary of Study ST002781

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001715. The data can be accessed directly via it's Project DOI: 10.21228/M81D9R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)
Study IDST002781
Study TitleLipidomics analysis of maternal obesity model - wild type
Study Summary10μl liver tissue of 11-12 weeks old male mice from various diet groups with various genetic background were homogenized and lipids were extracted for shotgun lipidomics experiments. Raw files were converted to .mzml files and imported into and analyzed by LipidXplorer software using custom mfql files to identify sample lipids and internal standards. For further data processing, absolute amounts were calculated using the internal standard intensities followed by the calculated mol% of the identified lipids. For wild-type C57BL/6JRcc mice, diet groups are CDm CDl CD, CDm CDl HFD, CDm HFDl HFD, HFDm CDl CD, HFDm CDl HFD, HFDm HFDl CD and HFDm CDl CD. m: maternal diet, l: lactation phase diet.
Institute
University of Bonn
DepartmentLIMES
LaboratoryMass Lab
Last NameMass
First NameElvira
AddressCarl-Troll-Str. 31, 53115 Bonn, Germany
Emailelvira.mass@uni-bonn.de
Phone+49 0228 / 73 6 28 48
Submit Date2023-07-02
Num Groups6
Total Subjects28
Analysis Type DetailMS(Dir. Inf.)
Release Date2023-08-01
Release Version1
Elvira Mass Elvira Mass
https://dx.doi.org/10.21228/M81D9R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Sample Preparation:

Sampleprep ID:SP002894
Sampleprep Summary:In this experiment, mouse livers were isolated and processed to extract lipids for analysis. The livers were homogenized and subjected to a series of centrifugation and phase separation steps to obtain the lipid samples. The extracted lipids were then prepared for further analysis by adding specific internal standards and a spray buffer, allowing for subsequent characterization and investigation.
Sampleprep Protocol Filename:Lipidomics_Notes.pdf
  logo