Summary of Study ST002782
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001715. The data can be accessed directly via it's Project DOI: 10.21228/M81D9R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002782 |
| Study Title | Lipidomics analysis of maternal obesity model - knock out |
| Study Summary | 10μl liver tissue of 11-12 weeks old male mice from various diet groups with various genetic background were homogenized and lipids were extracted for shotgun lipidomics experiments. Raw files were converted to .mzml files and imported into and analyzed by LipidXplorer software using custom mfql files to identify sample lipids and internal standards. For further data processing, absolute amounts were calculated using the internal standard intensities followed by the calculated mol% of the identified lipids. For Hif1a flox/flox or Hif1a flox/flox;LysMCre/+ mice with a C57BL/6JRcc background, diet groups contain CDm CD, CDm HFD, HFDm CD and HFDm HFD. m: maternal diet |
| Institute | University of Bonn |
| Department | LIMES |
| Laboratory | Mass Lab |
| Last Name | Mass |
| First Name | Elvira |
| Address | Carl-Troll-Str. 31, 53115 Bonn, Germany |
| elvira.mass@uni-bonn.de | |
| Phone | +49 0228 / 73 6 28 48 |
| Submit Date | 2023-07-03 |
| Num Groups | 8 |
| Total Subjects | 43 |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2023-08-01 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002895 |
| Sampleprep Summary: | In this experiment, mouse livers were isolated and processed to extract lipids for analysis. The livers were homogenized and subjected to a series of centrifugation and phase separation steps to obtain the lipid samples. The extracted lipids were then prepared for further analysis by adding specific internal standards and a spray buffer, allowing for subsequent characterization and investigation. |
| Sampleprep Protocol Filename: | Lipidomics_Notes.pdf |
