Summary of Study ST002873

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001795. The data can be accessed directly via it's Project DOI: 10.21228/M8PM7Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002873
Study Title	The ganglioside GM3 protects against abdominal aortic aneurysm (AAA) by suppressing ferroptosis
Study Summary	Background: Abdominal aortic aneurysm (AAA) is a potentially life-threatening condition, but approved medical therapies to prevent AAA progression and rupture are currently lacking. Sphingolipids metabolism disorders are associated with the occurrence and development of AAA. It has been discovered that ganglioside GM3, a sialic acid-containing type of glycosphingolipid, plays a protective role in atherosclerosis which is an important risk factor for AAA, but the potential contribution of GM3 to AAA development has not been investigated. Methods: We performed a metabolomics study to evaluated GM3 level in plasma of human AAA patients. We profiled GM3 synthase (ST3GAL5) expression in the mouse model of aneurysm and human AAA tissues through western blotting and immunofluorescence staining. RNA sequencing, proteomic analysis, affinity purification and mass spectrometry, surface plasmon resonance (SPR) analysis, and functional studies were used to dissect the molecular mechanism of GM3-regulating ferroptosis. We conditionally deleted and overexpressed St3gal5 in smooth muscle cells (SMCs) in vivo to investigate its role in AAA. Results: We found significantly reduced plasma levels of the GM3 in human AAA patients. GM3 content and ST3GAL5 expression were all decreased in abdominal aortic VSMCs in AAA patients and mouse model. RNA-sequencing analysis showed that ST3GAL5 silencing in human aortic SMCs (HASMCs) induced ferroptosis. Importantly, we showed that GM3 interacted directly with the extracellular domain of transferrin receptor 1 (TFR1), a cell membrane protein critical for cellular iron uptake, disrupted its interaction with holo-transferrin. SMC-specific St3gal5 knockout exacerbated iron accumulation at lesion sites and significantly promoted AAA development, while GM3 supplementation suppressed lipid peroxidation, reduced iron deposition in aortic VSMCs and markedly decreased AAA incidence. Conclusions: Together, these results suggest that GM3 dysregulation promotes ferroptosis of VSMCs in AAA. Furthermore, GM3 may constitute a new therapeutic target for the treatment of AAA.
Institute	Tianjin Medical University
Last Name	Li
First Name	Kan
Address	Tianjin Medical University, Tianjin, China., Tianjin, Tianjin, 300070, China
Email	likan1115@tmu.edu.cn
Phone	18853602951
Submit Date	2023-09-20
Raw Data Available	Yes
Raw Data File Type(s)	mzML, wiff
Analysis Type Detail	LC-MS
Release Date	2024-01-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002992
Sampleprep Summary:	Briefly, 50 μL plasma spiked with 10 µL internal standard mixture and 10 µL BHT was blended into 380 µL methanol (75%). MTBE (1 mL) was added, and the mixture was vortexed for 30 min. Phase separation was induced by adding 250 µL H₂O. After incubation for 10 min at room temperature, the samples were centrifuged at 14 000 g for 10 min. The upper phase was collected and dried using a nitrogen evaporator. Ultra-performance liquid chromatography BEH C18 columns (1.7 μm, 100 × 2.1 mm i.d.) were used for chromatographic separations.

Sampleprep ID:

SP002992

Sampleprep Summary:

Briefly, 50 μL plasma spiked with 10 µL internal standard mixture and 10 µL BHT was blended into 380 µL methanol (75%). MTBE (1 mL) was added, and the mixture was vortexed for 30 min. Phase separation was induced by adding 250 µL H₂O. After incubation for 10 min at room temperature, the samples were centrifuged at 14 000 g for 10 min. The upper phase was collected and dried using a nitrogen evaporator. Ultra-performance liquid chromatography BEH C18 columns (1.7 μm, 100 × 2.1 mm i.d.) were used for chromatographic separations.