Summary of Study ST001056
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000709. The data can be accessed directly via it's Project DOI: 10.21228/M81M4X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001056 |
Study Title | Evaluation of the short-term effects of the allelochemical umbelliferone on Triticum durum L. metabolism through GC-MS based untargeted metabolomics |
Study Summary | Allelopathy is a plant defense mechanism by which they protect themselves from competitive species using specialized biochemicals in the form of secretion or volatiles released to the environment. Though, umbelliferone is a well-known allelochemical, its mechanism of action in a short-term treatment is far from established. We used ≈ 10–days old wheat seedlings treated with104 µM umbelliferone over a time course experiment covering 6 times points, i.e., 0h, 6h, 12h, 24h, 48h, and 96h and compared the metabolomic changes to control (mock-treated) plants. Using gas chromatography mass-spectrometry (GC-MS) based metabolomics efforts, we collectively obtained quantitative data on 177 metabolites that were derivatized (either derivatized singly or multiple times) or not representing 139 non-redundant (unique) metabolites. Out of these 139 metabolites, 118 were associated with a unique HMDB identifier, while 113 were associated with a KEGG identifier. Relative quantification of these metabolites across the time-course of umbelliferone treatment, revealed 22 compounds (sugars, fatty acids, secondary metabolites, organic acids, and amino acids) that changed significantly (repeated measures ANOVA, P-value < 0.05) with time. Using multivariate partial least squares discriminant analysis (PLS-DA) we showed the grouping of samples based on time-course across the control and umbelliferone treated plants, whereas the metabolite-metabolite Pearson correlation revealed tightly formed clusters of umbelliferone-derived metabolites, fatty acids, amino acids, and carbohydrates. Also, time-course of umbelliferone treatment revealed, that phospho-L-serine, maltose, and dehydroquinic acid were the top three metabolites showing highest importance in discrimination among the time-points. The above indicate a system-wide changes induced by umbelliferone, through dysregulation of primary as well as specialized metabolism. |
Institute | Università Mediterranea di Reggio Calabria |
Department | Dipartimento AGRARIA |
Last Name | Araniti |
First Name | Fabrizio |
Address | Department AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, SNC I-89124, Reggio Calabria RC, Italy |
fabrizio.araniti@unirc.it | |
Phone | +39-09651694283 |
Submit Date | 2018-09-12 |
Num Groups | 3 |
Publications | In process |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | GC-MS |
Release Date | 2020-01-13 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR001378 |
Treatment Summary: | Seedlings (10 day old) were treated for 0 h (T0), 6 h (T1), 12 h (T2), 24 h (T3) and 96 h (T4) with nitrate (1 mM) and/ or umbelliferone (100 uM) |
Treatment Protocol Comments: | Durum weath seeds were directly sown in hydroponic systems the 11/March/2018 and allowed to establish for 10 days. Nutritional Regime:Plant were grown in hydroponic solution composed as follow: KNO3 (1 mM); MgSO4 (100 µM); CaSO4 (400 µM); KCl (5 µM); K2SO4 (200 µM); KH h2PO4 (175 µM); H h3BO3 (2.5 µM); MnSO4 (0.2 µM); ZnSO4 (0.2 µM); NaMoO4 (0.05 µM); CuSO4 (0.05 µM); Fe-EDTA (200 µM). Plant Watering Regime: plants were cropped in hydroponic solution daily changed |
Treatment: | Umbelliferone (Inhibitor: 100 uM), Time (0, 6, 12, 24, 48, 96 h) |
Treatment Compound: | Umbelliferone (Inhibitor: 100 uM) |
Treatment Route: | Roots- Hydropony |
Treatment Dose: | 100 uM |
Treatment Dosevolume: | Umbelliferone (Inhibitor: 100 uM), Nitrate (1 mM) |
Treatment Doseduration: | Time (0, 6, 12, 24, 48, 96 h) |
Treatment Vehicle: | Hoagland Solution |
Plant Growth Location: | Growth chambers belonging to the University Mediterranea of Reggio Calabria |
Plant Light Period: | 18h/6h light/dark |
Plant Humidity: | 60% Relative Humidity |
Plant Temp: | 25°C |
Plant Watering Regime: | See protocol comments |
Plant Nutritional Regime: | See protocol comments |
Plant Estab Date: | 2018-03-11 |
Plant Harvest Date: | 2018-03-25 |
Plant Growth Stage: | Seedlings collected at the formation of second true leaves |
Plant Metab Quench Method: | Immediately after collection snap frozen in liquid Nitrogen |
Plant Harvest Method: | Full plants harvested manually (5 plants for replicate) |
Plant Storage: | Snap frozen and powdered plant material stored at -80°C |