Summary of Study ST002606

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002606
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Kidney - Targeted Oxylipins
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Emory University
DepartmentBiochemistry
LaboratoryIntegrated Lipidomics and Metabolomics Core
Last NameOrtlund
First NameEric
Address1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
Emaileortlun@emory.edu
Phone(404) 727-5014
Submit Date2022-12-03
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Eric Ortlund Eric Ortlund
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001020
Project DOI:doi: 10.21228/M8V97D
Project Title:MoTrPAC
Project Summary:MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the effects of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. Preclinical and clinical studies will examine the systemic effects of endurance and resistance exercise across a range of ages and fitness levels by molecular probing of multiple tissues before and after acute and chronic exercise. This program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. The MoTrPAC program is supported by the NIH Common Fund and is managed by a trans-agency working group representing multiple NIH institutes and centers, led by the NIH Office of Strategic Coordination, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute on Aging, and National Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee: Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac Bioinformatics / Data Coordination Center.
Institute:MoTrPAC
Last Name:Ashley
First Name:Euan
Address:Falk Building CV267, 870 Quarry Road, Stanford, California 94305
Email:motrpac-data-deposition@lists.stanford.edu
Phone:(650) 725-1846

Subject:

Subject ID:SU002708
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26039890252015908Control 8 weeks of training or control time Female
SA26039990248015908Control 8 weeks of training or control time Female
SA26040090266015908Control 8 weeks of training or control time Female
SA26040190265015908Control 8 weeks of training or control time Female
SA26040290245015908Control 8 weeks of training or control time Female
SA26040390239015908Control 8 weeks of training or control time Male
SA26040490229015908Control 8 weeks of training or control time Male
SA26040590237015908Control 8 weeks of training or control time Male
SA26040690232015908Control 8 weeks of training or control time Male
SA26040790217015908Control 8 weeks of training or control time Male
SA260408QC_blank_65QC-Blank 0 hour -
SA260409QC_blank_4QC-Blank 0 hour -
SA260410QC_blank_68QC-Blank 0 hour -
SA260411QC_blank_69QC-Blank 0 hour -
SA260412QC_Standard_74QC-ExternalStandard 0 hour -
SA260413QC_Standard_75QC-ExternalStandard 0 hour -
SA260414QC_Standard_73QC-ExternalStandard 0 hour -
SA260415QC_Standard_72QC-ExternalStandard 0 hour -
SA260416QC_Standard_70QC-ExternalStandard 0 hour -
SA260417QC_Standard_71QC-ExternalStandard 0 hour -
SA260418QC_Standard_76QC-ExternalStandard 0 hour -
SA260419QC_Standard_77QC-ExternalStandard 0 hour -
SA260420QC_40QC-Pooled 0 hour -
SA260421QC_51QC-Pooled 0 hour -
SA260422QC_62QC-Pooled 0 hour -
SA260423QC_66QC-Pooled 0 hour -
SA260424QC_29QC-Pooled 0 hour -
SA260425QC_7QC-Pooled 0 hour -
SA260426QC_18QC-Pooled 0 hour -
SA260427QC_prerun_1QC-PreRun 0 hour -
SA260428QC_prerun_2QC-PreRun 0 hour -
SA260429QC_prerun_3QC-PreRun 0 hour -
SA260430QC_SRMQC-Reference 0 hour -
SA260431G_QC_1_63QC-Reference 0 hour -
SA260432G_QC_2_6QC-Reference 0 hour -
SA260433G_QC_1_5QC-Reference 0 hour -
SA260434G_QC_2_64QC-Reference 0 hour -
SA26043590560015908Training 1 week of training or control time Female
SA26043690564015908Training 1 week of training or control time Female
SA26043790567015908Training 1 week of training or control time Female
SA26043890559015908Training 1 week of training or control time Female
SA26043990571015908Training 1 week of training or control time Female
SA26044090422015908Training 1 week of training or control time Male
SA26044190430015908Training 1 week of training or control time Male
SA26044290421015908Training 1 week of training or control time Male
SA26044390423015908Training 1 week of training or control time Male
SA26044490426015908Training 1 week of training or control time Male
SA26044590585015908Training 2 weeks of training Female
SA26044690581015908Training 2 weeks of training Female
SA26044790578015908Training 2 weeks of training Female
SA26044890576015908Training 2 weeks of training Female
SA26044990587015908Training 2 weeks of training Female
SA26045090444015908Training 2 weeks of training Male
SA26045190439015908Training 2 weeks of training Male
SA26045290449015908Training 2 weeks of training Male
SA26045390450015908Training 2 weeks of training Male
SA26045490441015908Training 2 weeks of training Male
SA26045590412015908Training 4 weeks of training Female
SA26045690420015908Training 4 weeks of training Female
SA26045790406015908Training 4 weeks of training Female
SA26045890410015908Training 4 weeks of training Female
SA26045990416015908Training 4 weeks of training Female
SA26046090281015908Training 4 weeks of training Male
SA26046190280015908Training 4 weeks of training Male
SA26046290292015908Training 4 weeks of training Male
SA26046390283015908Training 4 weeks of training Male
SA26046490289015908Training 4 weeks of training Male
SA26046590254015908Training 8 weeks of training or control time Female
SA26046690251015908Training 8 weeks of training or control time Female
SA26046790267015908Training 8 weeks of training or control time Female
SA26046890259015908Training 8 weeks of training or control time Female
SA26046990258015908Training 8 weeks of training or control time Female
SA26047090223015908Training 8 weeks of training or control time Male
SA26047190222015908Training 8 weeks of training or control time Male
SA26047290225015908Training 8 weeks of training or control time Male
SA26047390227015908Training 8 weeks of training or control time Male
SA26047490218015908Training 8 weeks of training or control time Male
Showing results 1 to 77 of 77

Collection:

Collection ID:CO002701
Collection Summary:-
Sample Type:Kidney

Treatment:

Treatment ID:TR002718
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002714
Sampleprep Summary:purification of oxylipids with SPE column
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004278
Analysis type MS
Chromatography type Reversed phase
Chromatography system ExionLC AC
Column ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 Qtrap
Ion Mode NEGATIVE
Units ng/uL

Chromatography:

Chromatography ID:CH003184
Chromatography Summary:Lipids were separated by liquid chromatography with 20 minutes gradient
Methods Filename:pass1b_oxylipneg_etamidpos_methods.pdf
Instrument Name:ExionLC AC
Column Name:ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
Column Temperature:50C
Flow Gradient:t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @ 0.5 ml/min
Flow Rate:0.5 ml/min
Solvent A:100% water; 10 mM ammonium acetate
Solvent B:100% acetonitrile; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004025
Analysis ID:AN004278
Instrument Name:ABI Sciex 5500 Qtrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Multiple reaction monitoring mode (MRM)
Ion Mode:NEGATIVE
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