Summary of Study ST002842

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001766. The data can be accessed directly via it's Project DOI: 10.21228/M8FB1R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002842
Study TitleLeukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia (Fenofibrate)
Study SummaryTo investigate whether fenofibrate can restore hepatic lipid homeostasis, TgLC mice were fed with a regular chow or fenofibrate diet. Lipid metabolites in liver tissues were analyzed after Tamoxifen injection. A similar effect of fenofibrate on lipid metabolites was observed in C26 tumor-bearing mice.
Institute
Rutgers University
DepartmentDepartment of Radiation Oncology
Last NameHu
First NameWenwei
Address195 Little Albany St, New Brunswick, NJ 08901
Emailwh221@cinj.rutgers.edu
Phone7322356169
Submit Date2023-08-24
Study CommentsSame project as Study:ST002824
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-11-29
Release Version1
Wenwei Hu Wenwei Hu
https://dx.doi.org/10.21228/M8FB1R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001766
Project DOI:doi: 10.21228/M8FB1R
Project Title:Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia
Project Summary:We performed metabolomic analysis to measure polar and lipid metabolites in the serum from TgLC and TgL mice with Tamoxifen (TAM) injection. Mice under both fed and fasted conditions were included.
Institute:Rutgers University
Department:Department of Radiation Oncology
Last Name:Hu
First Name:Wenwei
Address:195 Little Albany St, New Brunswick, NJ 08901
Email:wh221@cinj.rutgers.edu
Phone:7322356169

Subject:

Subject ID:SU002952
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Treatment
SA307533c26-diet_7C26 + Feno diet Feno diet
SA307534c26-diet_1C26 + Feno diet Feno diet
SA307535c26-diet_8C26 + Feno diet Feno diet
SA307536c26-diet_2C26 + Feno diet Feno diet
SA307537c26-diet_9C26 + Feno diet Feno diet
SA307538c26-diet_10C26 + Feno diet Feno diet
SA307539c26_1C26 regular chow
SA307540c26_2C26 regular chow
SA307541c26_7C26 regular chow
SA307542c26_9C26 regular chow
SA307543c26_8C26 regular chow
SA307544c26_10C26 regular chow
SA307545TGLIF-diet_10TgLC + Feno diet Feno diet
SA307546TGLIF-diet_9TgLC + Feno diet Feno diet
SA307547TGLIF-diet_5TgLC + Feno diet Feno diet
SA307548TGLIF-diet_2TgLC + Feno diet Feno diet
SA307549TGLIF-diet_6TgLC + Feno diet Feno diet
SA307550TGLIF-diet_1TgLC + Feno diet Feno diet
SA307551TGLIF-diet_7TgLC + Feno diet Feno diet
SA307552TGLIF-diet_8TgLC + Feno diet Feno diet
SA307553TGLIF_6TgLC regular chow
SA307554TGLIF_4TgLC regular chow
SA307555TGLIF_7TgLC regular chow
SA307556TGLIF_5TgLC regular chow
SA307557TGLIF_9TgLC regular chow
SA307558TGLIF_3TgLC regular chow
SA307559TGLIF_10TgLC regular chow
SA307560TGLIF_8TgLC regular chow
Showing results 1 to 28 of 28

Collection:

Collection ID:CO002945
Collection Summary:TgLC mice were injected with Tamoxifen. Balb/c mice were injected with C26 cells. Mice were fed with a regular chow or fenofibrate diet. Liver was collected and then stored at -80 °C.
Sample Type:Liver

Treatment:

Treatment ID:TR002961
Treatment Summary:TgL and TgLC mice were injected with Tamoxifen. Balb/c mice were injected with C26 cells. Mice were fed with a regular chow or fenofibrate diet.

Sample Preparation:

Sampleprep ID:SP002958
Sampleprep Summary:For lipidomic metabolites extraction, 10 mg homogenized tissues were mixed with 120 μL methanol and 400 μL MTBE. After shaking for 6 minutes at 4 °C, 100 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further lipidomics analysis.

Combined analysis:

Analysis ID AN004651
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE
Units Ion counts

Chromatography:

Chromatography ID:CH003501
Chromatography Summary:Lipidomics method
Instrument Name:Thermo Vanquish
Column Name:Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
Column Temperature:55C
Flow Gradient:0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B
Flow Rate:0.2mL/min
Solvent A:90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Solvent B:75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004398
Analysis ID:AN004651
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Positive Ionization mode
Ion Mode:POSITIVE
Capillary Temperature:300
Ion Source Temperature:360
Spray Voltage:3500
Automatic Gain Control:3000000
Acquisition Parameters File:LCMS_Pos_RT_30min_55C_LongElution
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