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MB Sample ID: SA078302
| Local Sample ID: | Prednisolone-d0-P4 |
| Subject ID: | SU001204 |
| Subject Type: | Mammal |
| Subject Species: | Canis lupus familiaris |
| Taxonomy ID: | 9615 |
| Genotype Strain: | Beagle |
| Age Or Age Range: | 8 to 83 months |
| Weight Or Weight Range: | 12.2 to 18.9 kg |
| Gender: | Male and female |
| Animal Animal Supplier: | In-house breeding |
| Animal Feed: | Standard pellet/kibble maintenance diet |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001870 | AN001871 | AN001872 | AN001873 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1100 |
| Column | Agilent Zorbax RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um,95 Å) | Agilent Zorbax RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um,95 Å) | Waters Acquity BEH HILIC (100 x 2.1mm,1.7um,130 Å) | Agilent Zorbax Eclipse XDB C18 (150 x 3.0mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6460 QQQ | Agilent 6495 QQQ | Agilent 6490 QQQ | ABI Sciex 4000 QTrap |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | µmol/L | µmol/L | µmol/L | µmol/L |
Chromatography:
| Chromatography ID: | CH001351 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Agilent Zorbax RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um,95 Å) |
| Column Temperature: | 40 |
| Flow Gradient: | 20% B: 2 min, 20% - 60% B: 2 - 7 min, 60% - 100% B: 7 - 9 min, 20% B: 9 - 10.8 min |
| Flow Rate: | 0.4 mL/min |
| Sample Injection: | 2 µL |
| Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001352 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Agilent Zorbax RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um,95 Å) |
| Column Temperature: | 40 |
| Flow Gradient: | 20% B: 2 min, 20% - 60% B: 2 - 7 min, 60% - 100% B: 7 - 9 min, 20% B: 9 - 10.8 min |
| Flow Rate: | 0.4 mL/min |
| Sample Injection: | 1 µL |
| Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001353 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH HILIC (100 x 2.1mm,1.7um,130 Å) |
| Column Temperature: | 60 |
| Flow Gradient: | 99.9% B: 0 - 5 min; 40% B: 5 - 5.5 min; 10% B: 5.5 - 6.6 min and 99.9% B: 6.6 - 9.6 min (total run time 9.6 min) |
| Flow Rate: | 0.4 mL/min |
| Sample Injection: | 2 µL |
| Solvent A: | 50% water/50% acetonitrile; 25 mM ammonium formate, pH 4.6 |
| Solvent B: | 95% acetonitrile/5% water; 25 mM ammonium formate, pH 4.6 |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001354 |
| Instrument Name: | Agilent 1100 |
| Column Name: | Agilent Zorbax Eclipse XDB C18 (150 x 3.0mm,1.8um) |
| Column Temperature: | 25 |
| Flow Gradient: | Isocratic (25 min) |
| Flow Rate: | 0.128 mL/min |
| Sample Injection: | 10 µL |
| Solvent A: | 50% chloroform/50% methanol; 2 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
