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MB Sample ID: SA174465

Local Sample ID:146
Subject ID:SU001941
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606

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Combined analysis:

Analysis ID AN003021 AN003022
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Vanquish Vanquish
Column Kinetex XB-C18 (150 x 2.1mm,1.7um) Kinetex XB-C18 (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH002239
Chromatography Summary:10 μl of RBC extracts were injected and separated through a 3 min isocratic elution on a Kinetex XB-C18 column (150 × 2.1 mm i.d., 1.7 μm particle size – Phenomenex, Torrance, CA, USA) at 250 μl/min (mobile phase: 5% acetonitrile, 95% 18 mΩ H2O, 0.1% formic acid; column temperature: 25°C).
Instrument Name:Vanquish
Column Name:Kinetex XB-C18 (150 x 2.1mm,1.7um)
Column Temperature:25
Flow Gradient:isocratic
Flow Rate:250 ul/min
Solvent A:5% acetonitrile/95%water; 0.1% formic acid
Solvent B:isocratic
Chromatography Type:Reversed phase
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