Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA174465

Local Sample ID:	146
Subject ID:	SU001941
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP001947
Sampleprep Summary:	RBC and media samples were extracted at 1:10 and 1:20 dilutions, respectively, in ice-cold 5:3:2 MeOH:MeCN:H2O v/v/v, with vigorous vortexing at 4 degrees C for 30 min. The extraction buffer was supplemented with 40uM 3-13C lactate to allow absolute quantification of lactate isotopologues, including lactate generated via glycolysis ([1,2,3-13C3] enriched) vs. the pentose phosphate pathway ([2,3-13C2] enriched). Supernatants were clarified via centrifugation (10,000 g, 10 min, 4 C) then analyzed by UHPLC-MS.

Sampleprep ID:

SP001947

Sampleprep Summary:

RBC and media samples were extracted at 1:10 and 1:20 dilutions, respectively, in ice-cold 5:3:2 MeOH:MeCN:H2O v/v/v, with vigorous vortexing at 4 degrees C for 30 min. The extraction buffer was supplemented with 40uM 3-13C lactate to allow absolute quantification of lactate isotopologues, including lactate generated via glycolysis ([1,2,3-13C3] enriched) vs. the pentose phosphate pathway ([2,3-13C2] enriched). Supernatants were clarified via centrifugation (10,000 g, 10 min, 4 C) then analyzed by UHPLC-MS.