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MB Sample ID: SA239047
| Local Sample ID: | Z22 |
| Subject ID: | SU002487 |
| Subject Type: | Fish |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Genotype Strain: | wildtype and tango2bwh211 |
| Age Or Age Range: | 4 weeks |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003905 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity H-Class |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH002891 |
| Chromatography Summary: | Analysis was performed using a Thermo Q Exactive Plus coupled to a Waters Acquity H-Class LC. A 100 mm x 2.1 mm, 2.1 µm Waters BEH C18 column was used for separations. The following mobile phases were used: A- 60/40 ACN/H20 B- 90/10 IPA/ACN; both mobile phases contained 10 mM Ammonium Formate and 0.1% Formic Acid. A flow rate of 0.2 mL/minutes was used. Starting composition was 32% B, which increased to 40% B at 1 minute (held until 1.5 minutes) then 45% B at 4 minutes. This was increased to 50% B at 5 minutes, 60% B at 8 minutes, 70% B at 11 minutes, and 80% B at 14 minutes (held until 16 minutes). At 16 minutes the composition switched back to starting conditions (32% B) and was held for 4 minutes to re-equilibrate the column. Samples were analyzed in positive/negative switching ionization mode with top 5 data dependent fragmentation. |
| Instrument Name: | Waters Acquity H-Class |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Pressure: | 4220 psi |
| Column Temperature: | 45 |
| Flow Gradient: | Starting composition was 32% B, which increased to 40% B at 1 minute (held until 1.5 minutes) then 45% B at 4 minutes. This was increased to 50% B at 5 minutes, 60% B at 8 minutes, 70% B at 11 minutes, and 80% B at 14 minutes (held until 16 minutes). At 16 minutes the composition switched back to starting conditions (32% B) and was held for 4 minutes to re-equilibrate the column. |
| Flow Rate: | 0.2 mL/min |
| Injection Temperature: | 10 |
| Internal Standard: | Avanti EquiSplash mix |
| Sample Injection: | 5 µL |
| Solvent A: | 40% water/60% acetonitrile; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate |
| Capillary Voltage: | 3.50 KV |
| Running Voltage: | 3.50 KV |
| Weak Wash Solvent Name: | 90:10 Water:Acetontrile |
| Weak Wash Volume: | 300 µL |
| Strong Wash Solvent Name: | 1:1 Methanol:Isopropanol |
| Strong Wash Volume: | 50 µL |
| Sample Loop Size: | 10 µL |
| Sample Syringe Size: | 100 µL |
| Chromatography Type: | Reversed phase |
