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MB Sample ID: SA239047
| Local Sample ID: | Z22 |
| Subject ID: | SU002487 |
| Subject Type: | Fish |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Genotype Strain: | wildtype and tango2bwh211 |
| Age Or Age Range: | 4 weeks |
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Sample Preparation:
| Sampleprep ID: | SP002493 |
| Sampleprep Summary: | Control and tango2 mutant myofibers (4 weeks, n=5 each) were homogenized with 1 ml of MBTE. 300 µL of methanol with internal standard was added and samples were mixed for 10 minutes. 200 µL of water was added to facilitate phase separation. The extracts were centrifuged at 20,000 rcf for 10 minutes. The top layer was removed, dried and reconstituted in 150 µl of IPA for analysis. |
| Processing Method: | Liquid/Liquid Partition |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Liquid/Liquid Partition |
| Extract Cleanup: | Centrifugation (20,000 rcf for 10 min) |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 150 µL of IPA |
| Sample Derivatization: | none |
| Sample Spiking: | Spiked with 1.5 µg/mL of deuterated internal standards (Avanti EquiSplash mix) |
