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MB Sample ID: SA113196
| Local Sample ID: | 224_035 |
| Subject ID: | SU001464 |
| Subject Type: | Mammal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Gender: | Male |
| Species Group: | Mammals |
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Sample Preparation:
| Sampleprep ID: | SP001472 |
| Sampleprep Summary: | Whole blood samples were thawed on ice and subjected to a methanol-chloroform precipitation. Briefly, samples were thawed in an ice-water bath after which 500 μL of blood was transferred to a microcentrifuge tube and 1ml of a 1:1 methanol-chloroform solution was added. Samples were sonicated for 2 minutes at 4°C, then incubated at -20°C for 20 minutes, and then centrifuged (13,400g at 4°C for 30 minutes). The aqueous supernatant was transferred to a new microcentrifuge tube and dried by lyophilization. Samples were then resuspended in 600 μL of 50mM sodium phosphate buffer in D2O for NMR analysis. |
| Processing Storage Conditions: | On ice |
