Summary of Study ST001369
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000922. The data can be accessed directly via it's Project DOI: 10.21228/M8HH5M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001369 |
| Study Title | Grass pollen sublingual immunotherapy treatment induces transcriptomic and metabolic changes due to AIT treatment |
| Study Summary | 47 patients were enrolled in a double-blind, placebo-controlled, multicenter trial using with GRAZAX® (Phleum pretense) during 2 years of therapy (T2). Immunological assays such as sIgE, sIgG4 and ISAC were carried out to the 31 patients who finished the trial. Additionally, serum and PBMCs samples from these samples were analyzed by metabolomics and transcriptomics, respectively. Based on their sensitization level, 22 patients were grouped in Mono and Poli groups, excluding epithelial allergic patients. Individuals were studied based on their treatment in Active and Placebo and their sensitization level. For metabolomics, samples were analyzed by Liquid and Gas Chromatography coupled to Mass Spectrometry (LC-MS and GC-MS, respectively). |
| Institute | Institute of Applied Molecular Medicine;The Centre of Metabolomics and Bioanalysis |
| Last Name | Obeso Montero |
| First Name | David |
| Address | Avda. Monteprincipe s/n 28668 Boadilla del Monte, Madrid, España |
| david.obesomontero@beca.ceu.es | |
| Phone | Tlf: 91 372 47 00 ext. 4662 |
| Submit Date | 2020-03-27 |
| Num Groups | 2 main groups: Active and Placebo, and 2 subgroups: Monosensitized and Polisensitized patients. |
| Total Subjects | 22 |
| Study Comments | https://www.ceu.es;http://www.metabolomica.uspceu.es |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2020-09-29 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002283 | AN002284 | AN002285 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | GC |
| Chromatography system | Agilent 1200 | Agilent 1200 | Agilent 7890A |
| Column | Discovery HS C18 (150 x 2.1mm,3.0um) Supelco,Sigma Aldrich,Germany),with a guard Discovery HS C18 (2 cm x 2.1mm,3um; Supelco,Sigma Aldrich,Germany), | Discovery HS C18 (150 x 2.1mm,3.0um) Supelco,Sigma Aldrich,Germany) | GC-Column DB5-MS (30 m length, 0.25 mm i.d., 0.25 μm film 95% dimethyl/5% diphenylpolysiloxane) with an integrated precolumn (10 m J&W, Agilent) |
| MS Type | ESI | ESI | EI |
| MS instrument type | QTOF | QTOF | Single quadrupole |
| MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF | Agilent 5975C |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | peak area | peak area | peak area |
