Summary of Study ST001369
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000922. The data can be accessed directly via it's Project DOI: 10.21228/M8HH5M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001369 |
| Study Title | Grass pollen sublingual immunotherapy treatment induces transcriptomic and metabolic changes due to AIT treatment |
| Study Summary | 47 patients were enrolled in a double-blind, placebo-controlled, multicenter trial using with GRAZAX® (Phleum pretense) during 2 years of therapy (T2). Immunological assays such as sIgE, sIgG4 and ISAC were carried out to the 31 patients who finished the trial. Additionally, serum and PBMCs samples from these samples were analyzed by metabolomics and transcriptomics, respectively. Based on their sensitization level, 22 patients were grouped in Mono and Poli groups, excluding epithelial allergic patients. Individuals were studied based on their treatment in Active and Placebo and their sensitization level. For metabolomics, samples were analyzed by Liquid and Gas Chromatography coupled to Mass Spectrometry (LC-MS and GC-MS, respectively). |
| Institute | Institute of Applied Molecular Medicine;The Centre of Metabolomics and Bioanalysis |
| Last Name | Obeso Montero |
| First Name | David |
| Address | Avda. Monteprincipe s/n 28668 Boadilla del Monte, Madrid, España |
| david.obesomontero@beca.ceu.es | |
| Phone | Tlf: 91 372 47 00 ext. 4662 |
| Submit Date | 2020-03-27 |
| Num Groups | 2 main groups: Active and Placebo, and 2 subgroups: Monosensitized and Polisensitized patients. |
| Total Subjects | 22 |
| Study Comments | https://www.ceu.es;http://www.metabolomica.uspceu.es |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2020-09-29 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002283 | AN002284 | AN002285 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | GC |
| Chromatography system | Agilent 1200 | Agilent 1200 | Agilent 7890A |
| Column | Discovery HS C18 (150 x 2.1mm,3.0um) Supelco,Sigma Aldrich,Germany),with a guard Discovery HS C18 (2 cm x 2.1mm,3um; Supelco,Sigma Aldrich,Germany), | Discovery HS C18 (150 x 2.1mm,3.0um) Supelco,Sigma Aldrich,Germany) | GC-Column DB5-MS (30 m length, 0.25 mm i.d., 0.25 μm film 95% dimethyl/5% diphenylpolysiloxane) with an integrated precolumn (10 m J&W, Agilent) |
| MS Type | ESI | ESI | EI |
| MS instrument type | QTOF | QTOF | Single quadrupole |
| MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF | Agilent 5975C |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | peak area | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001677 |
| Chromatography Summary: | For LC-MS, 10 μL of sample were injected into a Discovery HS C18 column (2.1 mm × 150 mm, 3.0 μm; Supelco, Sigma Aldrich, Germany), with a guard column Discovery® HS C18 (2 cm × 2.1 mm, 3 μm; Supelco, Sigma Aldrich, Germany), both maintained at 40 °C. The flow rate was set at 0.6 mL/min. The gradient elution involved a mobile phase consisting of (A) 0.1% formic acid (FA) in water and (B) 0.1% FA in acetonitrile (ACN). The initial condition was set at 25% B, increasing to 95% B in 35 min, followed by re-equilibration for 1 min, finally it was held for 9 min in initial conditions. Flow rate was set at 0.6 mL/min, and 10 μL of samples were injected. The electrospray source ionization (ESI) data were acquired in positive and negative ion mode, respectively. The capillary voltage was set at 3,500 for ESI (+) and 4,000V for ESI (−). The drying gas flow rate was 10.5 L/min at 330 °C and gas nebulizer at 52 psi; fragmentor voltage was 175 V; skimmer and octopole radio frequency voltage (OCT RF Vpp) were set to 65 and 750 V. Data were collected in the centroid mode at a scan rate of 1.2 spectra per second. Mass spectrometry detection was performed in full scan from 100 to 1200 m/z for both, positive and negative ESI mode. The reference m/z ions were purine (121.0508) and HP-0921 (922.0097) for ESI (+), whereas TFA NH4 (119.0363) and HP-0921 (966.0007) for ESI (−). These masses were continuously infused into the system to allow constant mass correction. Samples were analyzed in separate runs. |
| Instrument Name: | Agilent 1200 |
| Column Name: | Discovery HS C18 (150 x 2.1mm,3.0um) Supelco,Sigma Aldrich,Germany),with a guard Discovery HS C18 (2 cm x 2.1mm,3um; Supelco,Sigma Aldrich,Germany), |
| Column Temperature: | 40 |
| Flow Gradient: | The initial condition was set at 25% B, increasing to 95% B in 35 min, followed by re-equilibration for 1 min, finally it was held for 9 min in initial conditions |
| Flow Rate: | 0.6ml/min |
| Injection Temperature: | 4 |
| Sample Injection: | 10 μL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 45 min |
| Capillary Voltage: | 3500 for ESI (+) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001678 |
| Chromatography Summary: | For LC-MS, 10 μL of sample were injected into a Discovery HS C18 column (2.1 mm × 150 mm, 3.0 μm; Supelco, Sigma Aldrich, Germany), with a guard column Discovery® HS C18 (2 cm × 2.1 mm, 3 μm; Supelco, Sigma Aldrich, Germany), both maintained at 40 °C. The flow rate was set at 0.6 mL/min. The gradient elution involved a mobile phase consisting of (A) 0.1% formic acid (FA) in water and (B) 0.1% FA in acetonitrile (ACN). The initial condition was set at 25% B, increasing to 95% B in 35 min, followed by re-equilibration for 1 min, finally it was held for 9 min in initial conditions. Flow rate was set at 0.6 mL/min, and 10 μL of samples were injected. The electrospray source ionization (ESI) data were acquired in positive and negative ion mode, respectively. The capillary voltage was set at 3,500 for ESI (+) and 4,000V for ESI (−). The drying gas flow rate was 10.5 L/min at 330 °C and gas nebulizer at 52 psi; fragmentor voltage was 175 V; skimmer and octopole radio frequency voltage (OCT RF Vpp) were set to 65 and 750 V. Data were collected in the centroid mode at a scan rate of 1.2 spectra per second. Mass spectrometry detection was performed in full scan from 100 to 1200 m/z for both, positive and negative ESI mode. The reference m/z ions were purine (121.0508) and HP-0921 (922.0097) for ESI (+), whereas TFA NH4 (119.0363) and HP-0921 (966.0007) for ESI (−). These masses were continuously infused into the system to allow constant mass correction. Samples were analyzed in separate runs. |
| Instrument Name: | Agilent 1200 |
| Column Name: | Discovery HS C18 (150 x 2.1mm,3.0um) Supelco,Sigma Aldrich,Germany) |
| Column Temperature: | 40 |
| Flow Gradient: | The initial condition was set at 25% B, increasing to 95% B in 35 min, followed by re-equilibration for 1 min, finally it was held for 9 min in initial conditions |
| Flow Rate: | 0.6ml/min |
| Injection Temperature: | 4 |
| Sample Injection: | 10 μL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 45 min |
| Capillary Voltage: | 4000V for ESI (−) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001679 |
| Chromatography Summary: | GC-MS analysis was performed by a GC system (Agilent Technologies 7890A) equipped with an autosampler (Agilent 7693) coupled to a mass spectrometer with triple-Axis detector (5975C, Agilent). Two microliters (2 μL) of the derivatized sample were injected through a GC-Column DB5-MS (30 m length, 0.25 mm i.d., 0.25 μm film 95% dimethyl/5% diphenylpolysiloxane) with an integrated precolumn (10 m J&W, Agilent). Carrier gas (He) flow rate was set at 1 mL/min and injector temperature at 250 °C. Split ratio was fixed from 1:5 to 1:10 with 3 to 10 mL/min. He split flow into a Restek 20782 (Bellefonte, PA, USA) deactivated glass-wool split liner. The temperature gradient was programmed as follows: the initial oven temperature was set at 60 ºC (held for 1 min), increased to 325 ºC at 10 ºC/min rate (within 26.5 min) and hold 325 ºC for 10 min. The total run time was 37.5 min. A cool-down period was applied for 10 min before the next injection. Detector transfer line, filament source and the quadrupole temperature were set at 280 ºC, 230 ºC and 150 ºC, respectively. MS detection was performed with electron ionization (EI) mode at -70 eV. The mass spectrometer was operated in scan mode over a mass range of m/z 50-600 at a rate of 2.7 scan/s. Several IS injections, a standard mix of fatty acid methyl esters (FAME C8-C30), extraction blanks and 3 QCs samples were injected at the beginning of analysis, following QCs injections every 5 experimental samples and 1 QC injection at the end of worklist. |
| Instrument Name: | Agilent 7890A |
| Column Name: | GC-Column DB5-MS (30 m length, 0.25 mm i.d., 0.25 μm film 95% dimethyl/5% diphenylpolysiloxane) with an integrated precolumn (10 m J&W, Agilent) |
| Column Temperature: | As Oven |
| Injection Temperature: | 250 |
| Internal Standard: | Standard mix of fatty acid methyl esters (FAME C8-C30) |
| Sample Injection: | 2 μL |
| Analytical Time: | 37.5 min |
| Oven Temperature: | The initial oven temperature was set at 60 ºC (held for 1 min), increased to 325 ºC at 10 ºC/min rate (within 26.5 min) and hold 325 ºC for 10 min. |
| Transferline Temperature: | 280 |
| Chromatography Type: | GC |
